Xu Tong-kuan, Shen Xing-hai, Gao Hong-cheng
College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2005 Jun;25(6):857-60.
A substituted 3H-indole quaternary ammonium molecule was designed and synthesized using hexamethylphosphoramide (HMPA) as a solvent. The products were purified and characterized by IR, 1H-NMR, MS and elemental analysis. The binding reaction of this compound with bovine serum albumin (BSA) in aqueous solution was studied using fluorescence. Their binding constant is Ka = 1.995 x 10(5) dm3 x mol(-1) and the binding site number is n = 1.12. It is confirmed that the combination is a single static quenching process.
以六甲基磷酰胺（HMPA）为溶剂设计并合成了一种取代的3H-吲哚季铵分子。产物经红外光谱（IR）、核磁共振氢谱（1H-NMR）、质谱（MS）和元素分析进行纯化和表征。利用荧光光谱研究了该化合物与水溶液中牛血清白蛋白（BSA）的结合反应。它们的结合常数为Ka = 1.995×10(5) dm3·mol(-1)，结合位点数为n = 1.12。证实该结合为单一的静态猝灭过程。
