基于减毒活疫苗生产株SA14-14-2 cDNA模板构建感染性日本脑炎病毒克隆
[Construction of infectious Japanese encephalitis virus clone based on the cDNA template of the attenuated live vaccine production strain SA14-14-2].
作者信息
Zeng Ming, Jia Li-li, Yu Yong-xin, Dong Guan-mu, Liu Wen-xue, Wang Zhi-wei, Li De-fu
机构信息
National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2005 Mar;19(1):9-11.
OBJECTIVE
To construct infectious Japanese encephalitis virus (JEV) based on the in vitro-ligated cDNA template of the vaccine strain SA14-14-2, and identify the virus.
METHODS
Full-length genomic cDNA of JEV SA14-14-2 strain was ligated and then RNA was transcribed in vitro, the infective virus was obtained by transfecting the RNA into Vero cells and identified.
RESULTS
The infective clone of JEV was constructed, the virulence was weaker than the wild virus.
CONCLUSION
It was possible to construct infectious clone from the production strain of live attenuated Japanese B encephalitis vaccine.
目的
基于疫苗株SA14 - 14 - 2的体外连接cDNA模板构建感染性日本脑炎病毒（JEV），并对该病毒进行鉴定。
方法
将JEV SA14 - 14 - 2株全长基因组cDNA进行连接，然后体外转录RNA，将RNA转染至Vero细胞获得感染性病毒并进行鉴定。
结果
构建了JEV感染性克隆，其毒力低于野生病毒。
结论
从减毒活日本脑炎疫苗生产毒株构建感染性克隆是可行的。
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