Wan Su-jun, Li Jian-nong, Zhao Hong, Wang Li-xia, Huang Xia-zhen, Zhu Yan, Chen Hong-shan
Guang An Men Hospital, The Chinese Academy of Traditional Chinese Medicine, Beijing 100053, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2005 Mar;19(1):77-9.
To investigate the effect of Chinese herbal medicine Xin-kang oral liquid on interferon (IFN)-induction and its antiviral activity in Coxsackievirus B3 virus strain (CVB3) infected mice.
The Xin-kang oral liquid was given orally to mice two days prior to the challenge of CVB3 virus to induce myocarditis. Two dosages of Xin-kang oral liquid crude herbal medicine 30 g x kg(-1) x d(-1) and 12 g x kg(-1) x d(-1) were given to the mice of different treatment groups respectively, sterilized water was given to the mice of virus control group. IFN-alpha 10(6) U x kg(-1) x d(-1) S.C was given to the infected mice as positive drug control group. The mice were sacrificed on 5th, 10th and 20th day of infection for evaluation, the levels of serum interferon (IFN) were titrated with vesicular stomatitis virus (VSV) and cardiac tissue was fixed and sectioned. The quantitative histological changes at various stages of myocarditis were observed.
In the infected mice fed with 30 g x kg(-1) x d(-1) or 12 g x kg(-1) x d(-1) of Xin-kang oral liquid orally for 5, 10 and 20 days, the mean titer of serum IFN of Xin-Kang oral liquid treated group was markedly higher (29.3 U/0.1 ml) than that of virus control group (12.6 U/0.1 ml). The level of serum IFN in IFN treated positive control mice was lower than that of Xin-kang treatment groups. The histological examination showed extensive myocardial necrosis and cellular infiltration in virus control group, but necrosis and cellular infiltration were less severe in Xin-kang treatment goups of mice. It is demonstrated that there were close correlation between the degree of myocardial lesions and the level of IFN-induction in treated mice.
Xin-kang oral liquid could facilitate the induction of endogenous interferon that exerted its antiviral activity in CVB3 infected mice. This can help us to understand better the mechanism of anti-CVB3 effect of Xin-Kang oral liquid.
探讨中药心康口服液对柯萨奇B3病毒株（CVB3）感染小鼠干扰素（IFN）诱导作用及其抗病毒活性。
在CVB3病毒攻击小鼠前两天口服心康口服液以诱导心肌炎。不同治疗组小鼠分别给予两种剂量的心康口服液粗药，即30 g·kg⁻¹·d⁻¹和12 g·kg⁻¹·d⁻¹，病毒对照组小鼠给予灭菌水。感染小鼠皮下注射IFN-α 10⁶ U·kg⁻¹·d⁻¹作为阳性药物对照组。在感染后第5、10和20天处死小鼠进行评估，用水疱性口炎病毒（VSV）滴定血清干扰素（IFN）水平，并固定心脏组织切片。观察心肌炎各阶段的定量组织学变化。
口服30 g·kg⁻¹·d⁻¹或12 g·kg⁻¹·d⁻¹心康口服液5、10和20天的感染小鼠，心康口服液治疗组血清IFN平均滴度（29.3 U/0.1 ml）明显高于病毒对照组（12.6 U/0.1 ml）。IFN治疗的阳性对照小鼠血清IFN水平低于心康治疗组。组织学检查显示病毒对照组有广泛的心肌坏死和细胞浸润，但心康治疗组小鼠的坏死和细胞浸润较轻。结果表明，治疗小鼠心肌损伤程度与IFN诱导水平密切相关。
心康口服液可促进内源性干扰素的诱导，在CVB3感染小鼠中发挥抗病毒活性。这有助于我们更好地理解心康口服液抗CVB3作用的机制。
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