[中药心康口服液对柯萨奇病毒B3感染小鼠中CVB3 RNA复制的抑制作用]
[Inhibitory effect of Chinese herbal medicine Xin-Kang oral liquid on replication of CVB3 RNA in Coxsackievirus B3 infected mice].
作者信息
Wan Su-Jun, Yang Fan, Huang Xia-Zhen
机构信息
Guang An Men Hospital, The Chinese Academy of Traditional Chinese Medicine, Beijing, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2007 Sep;21(3):238-40.
OBJECTIVE
To investigate the CVB3 RNA concentration in myocardial tissues and inhibitory effect of Chinese herbal medicine Xin-Kang oral liquid on viral RNA replication in Coxsackievirus B3 infected myocardium.
METHODS
The total RNA was extracted from the infected murine myocardium, the reverse transcription-polymerase chain reaction (RT-PCR) was performed to detect enterovirus RNA concentration in infected mice with Coxsackievirus B3 gene band. We used "Gel works system" to scan the electrophoresis image to detect CVB3 gene band.
RESULTS
The mean concentration of myocardial CVB3 RNA of Xin-Kang oral liquid treated groups was markedly lower than that of virus control group (P>0.01), but the CVB3 RNA in myocardial tissues has not been destroyed by Xin-Kang oral liquid feed in different phase.
CONCLUSION
Chinese herbal medicine Xin-Kang oral liquid could inhibit CVB3 RNA replication in myocardial tissue, but it did not destruct the virus.
目的
探讨柯萨奇B3型病毒（CVB3）感染心肌组织中病毒RNA的含量以及中药心康口服液对感染心肌中病毒RNA复制的抑制作用。
方法
从感染病毒的小鼠心肌组织中提取总RNA，采用逆转录聚合酶链反应（RT-PCR）检测感染柯萨奇B3型病毒小鼠肠道病毒RNA的含量，并以柯萨奇B3型病毒基因条带表示。应用“Gel works系统”扫描电泳图像检测CVB3基因条带。
结果
心康口服液治疗组心肌CVB3 RNA平均含量明显低于病毒对照组（P>0.01），但不同时期心康口服液灌胃未破坏心肌组织中的CVB3 RNA。
结论
中药心康口服液可抑制心肌组织中CVB3 RNA的复制，但未破坏病毒。
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