Woo Han Young, Korystov Dmitry, Mikhailovsky Alexander, Nguyen Thuc-Quyen, Bazan Guillermo C
Mitsubishi Chemical Center for Advanced Materials, Department of Materials, Institute for Polymers and Organic Solids, University of California, Santa Barbara, California 93106, USA.
J Am Chem Soc. 2005 Oct 12;127(40):13794-5. doi: 10.1021/ja054911q.
Addition of the surfactant sodium dodecyl sulfate at concentrations above the critical micelle concentration increases the fluorescence quantum yield and the two-photon absorption cross-section of charged [2.2]paracyclophane chromophores containing pairs of D-pi-D chromophores. The resulting spectra in the micellar solutions are very similar to those obtained for neutral isostructural analogues in toluene. The measured etadelta values are 1300 GM for 1C and 1920 GM for 2C, which are comparable or larger to those in toluene. These results highlight possible misleading interpretation of two-photon-induced emission for evaluating the concentration of labeled substrates used in two-photon microscopy and provide guidelines for designing molecular structures with optimized two-photon action cross-sections in water.
在高于临界胶束浓度的浓度下添加表面活性剂十二烷基硫酸钠，会增加含有D-π-D发色团对的带电[2.2]对环芳发色团的荧光量子产率和双光子吸收截面。胶束溶液中得到的光谱与在甲苯中中性同构类似物得到的光谱非常相似。测得的1C的δ值为1300 GM，2C的δ值为1920 GM，与甲苯中的值相当或更大。这些结果突出了在评估双光子显微镜中使用的标记底物浓度时，双光子诱导发射可能存在的误导性解释，并为在水中设计具有优化双光子作用截面的分子结构提供了指导。
