雌激素受体相关受体α、β和γ在卵巢癌细胞中的作用

[Role of estrogen receptor-related receptors alpha, beta and gamma in ovarian cancer cells].

作者信息

Sun Peng-ming, Wei Li-hui, Sehouli Jalid, Denkert Carsten, Zhao Dan, Gao Min, Sun Xiu-li, Litchtenegger Werner

机构信息

Department Of Gynecology, People's Hospital, Peking University, Beijing 100044, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2005 Aug;40(8):544-8.

PMID:16202294

Abstract

OBJECTIVE

To investigate the role of human estrogen receptor-related receptors (ERRs) in ovarian cancer.

METHODS

A green fluorescent protein (GFP) reporter plasmid of ERRalpha was constructed and transfected into ovarian cancer cell lines SKOV3 and OVCAR3. RT-PCR was used to analyze the expression of ERRalpha, ERRbeta, and ERRgamma mRNA in 33 primary ovarian cancer samples and 12 normal ovarian epithelial cells. Progression-free survival time and overall survival time of patients with different expression of ERRs were analyzed by Kaplan-Meier method.

RESULTS

GFP-ERRalpha fusion protein showed ERRalpha expressed in the ovarian cancer cell nucleus. Expressions of the ERRalpha and ERRgamma were increased in the ovarian cancers (58% and 48%) compared with the normal ovarian tissues (P < 0.05); ERRbeta was weakly expressed in the ovarian cancer (9%) as well as in the normal tissues (0). Expression of ERRalpha was positively correlated with the International Federation of Gynecology and Obstetrics (FIGO) stage (r = 0.639, P = 0.017) and cell grading (r = 0.520, P = 0.022). Survival analysis showed that ERRalpha positive group had a poorer overall survival (19.0 months vs. 31.5 months, P < 0.05) and ERRgamma positive group had a longer progression-free survival (18.0 months vs. 13.5 months, P < 0.05).

CONCLUSIONS

ERRalpha chiefly expresses in the cell nucleus. ERRalpha and ERRgamma may play important roles in ovarian cancer.

摘要

目的

研究人类雌激素受体相关受体（ERRs）在卵巢癌中的作用。

方法

构建ERRα的绿色荧光蛋白（GFP）报告质粒，并转染至卵巢癌细胞系SKOV3和OVCAR3。采用逆转录聚合酶链反应（RT-PCR）分析33例原发性卵巢癌样本和12例正常卵巢上皮细胞中ERRα、ERRβ和ERRγ mRNA的表达。采用Kaplan-Meier法分析ERRs不同表达患者的无进展生存期和总生存期。

结果

GFP-ERRα融合蛋白显示ERRα在卵巢癌细胞核中表达。与正常卵巢组织相比，卵巢癌中ERRα和ERRγ的表达增加（分别为58%和48%）（P<0.05）；ERRβ在卵巢癌（9%）和正常组织（0）中均弱表达。ERRα的表达与国际妇产科联盟（FIGO）分期（r=0.639，P=0.017）和细胞分级（r=0.520，P=0.022）呈正相关。生存分析显示，ERRα阳性组的总生存期较差（19.0个月对31.5个月，P<0.05），ERRγ阳性组的无进展生存期较长（18.0个月对13.5个月，P<0.05）。