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Comparison of rectoanal mucosal swab cultures and fecal cultures for determining prevalence of Escherichia coli O157:H7 in feedlot cattle.用于确定饲养场牛中大肠杆菌O157:H7流行率的直肠肛门黏膜拭子培养与粪便培养的比较
Appl Environ Microbiol. 2005 Oct;71(10):6431-3. doi: 10.1128/AEM.71.10.6431-6433.2005.
2
Rectoanal mucosal swab culture is more sensitive than fecal culture and distinguishes Escherichia coli O157:H7-colonized cattle and those transiently shedding the same organism.直肠肛门黏膜拭子培养比粪便培养更敏感，且能区分感染大肠杆菌O157:H7的牛和短暂排出该菌的牛。
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Rectoanal junction colonization of feedlot cattle by Escherichia coli O157:H7 and its association with supershedders and excretion dynamics.育肥牛直肠肛管结合部大肠杆菌O157:H7的定植及其与超级排菌者和排泄动态的关系。
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Comparison of cultures from rectoanal-junction mucosal swabs and feces for detection of Escherichia coli O157 in dairy heifers.比较直肠肛管交界黏膜拭子和粪便培养物以检测奶牛小母牛中的大肠杆菌O157 。
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Influence of processed grains on fecal pH, starch concentration, and shedding of Escherichia coli O157 in feedlot cattle.加工谷物对育肥牛粪便pH值、淀粉浓度及大肠杆菌O157排泄的影响。
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本文引用的文献

1
Epidemiology of Escherichia coli O157 in Feedlot Cattle.饲养场牛群中大肠杆菌O157的流行病学
J Food Prot. 1997 May;60(5):462-465. doi: 10.4315/0362-028X-60.5.462.
2
Rectal carriage of enterohemorrhagic Escherichia coli O157 in slaughtered cattle.屠宰牛中肠出血性大肠杆菌O157的直肠携带情况
Appl Environ Microbiol. 2005 Jan;71(1):93-7. doi: 10.1128/AEM.71.1.93-97.2005.
3
Intermittent and persistent shedding of Escherichia coli O157 in cohorts of naturally infected calves.自然感染小牛群体中大肠杆菌O157的间歇性和持续性排菌
J Appl Microbiol. 2004;97(5):1045-53. doi: 10.1111/j.1365-2672.2004.02390.x.
4
Effect of forage or grain diets with or without monensin on ruminal persistence and fecal Escherichia coli O157:H7 in cattle.含或不含莫能菌素的草料或谷物日粮对牛瘤胃持久性及粪便中大肠杆菌O157:H7的影响
Appl Environ Microbiol. 2004 Sep;70(9):5336-42. doi: 10.1128/AEM.70.9.5336-5342.2004.
5
Rectal administration of Escherichia coli O157:H7: novel model for colonization of ruminants.大肠杆菌O157:H7的直肠给药：反刍动物定植的新模型。
Appl Environ Microbiol. 2004 Aug;70(8):4588-95. doi: 10.1128/AEM.70.8.4588-4595.2004.
6
Pulsed field gel electrophoresis of related Escherichia coli O157 isolates associated with beef cattle and comparison with unrelated isolates from animals, meats and humans.与肉牛相关的大肠杆菌O157分离株的脉冲场凝胶电泳分析及与来自动物、肉类和人类的非相关分离株的比较
Int J Food Microbiol. 2004 Apr 15;92(2):161-9. doi: 10.1016/j.ijfoodmicro.2003.09.007.
7
Longitudinal study of fecal shedding of Escherichia coli O157:H7 in feedlot cattle: predominance and persistence of specific clonal types despite massive cattle population turnover.饲养场牛群中大肠杆菌O157:H7粪便排菌的纵向研究：尽管牛群数量大量更替，但特定克隆型仍占主导并持续存在。
Appl Environ Microbiol. 2004 Jan;70(1):377-84. doi: 10.1128/AEM.70.1.377-384.2004.
8
Rectoanal mucosal swab culture is more sensitive than fecal culture and distinguishes Escherichia coli O157:H7-colonized cattle and those transiently shedding the same organism.直肠肛门黏膜拭子培养比粪便培养更敏感，且能区分感染大肠杆菌O157:H7的牛和短暂排出该菌的牛。
J Clin Microbiol. 2003 Nov;41(11):4924-9. doi: 10.1128/JCM.41.11.4924-4929.2003.
9
Escherichia coli O157 in feedlot cattle feces and water in four major feeder-cattle states in the USA.美国四个主要育肥牛州的饲养场牛粪和水中的大肠杆菌O157
Prev Vet Med. 2003 Oct 15;61(2):127-35. doi: 10.1016/s0167-5877(03)00166-1.
10
Evaluation of pulsed-field gel electrophoresis as a tool for determining the degree of genetic relatedness between strains of Escherichia coli O157:H7.评估脉冲场凝胶电泳作为确定大肠杆菌O157:H7菌株间遗传相关性程度的工具。
J Clin Microbiol. 2003 May;41(5):1843-9. doi: 10.1128/JCM.41.5.1843-1849.2003.

用于确定饲养场牛中大肠杆菌O157:H7流行率的直肠肛门黏膜拭子培养与粪便培养的比较

Comparison of rectoanal mucosal swab cultures and fecal cultures for determining prevalence of Escherichia coli O157:H7 in feedlot cattle.

作者信息

Greenquist M A, Drouillard J S, Sargeant J M, Depenbusch B E, Shi Xiaorong, Lechtenberg K F, Nagaraja T G

机构信息

Department of Diagnostic Medicine/Pathobiology, 305 Coles Hall, Kansas State University, Manhattan, KS 66506-5606, USA.

出版信息

Appl Environ Microbiol. 2005 Oct;71(10):6431-3. doi: 10.1128/AEM.71.10.6431-6433.2005.

DOI:10.1128/AEM.71.10.6431-6433.2005
PMID:16204574
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1265949/
Abstract

We compared fecal samples with samples collected with rectoanal mucosa swabs (RAMS) to determine the prevalence of Escherichia coli O157 in feedlot cattle (n = 747). Escherichia coli O157 was detected in 9.5% of samples collected with RAMS and 4.7% of samples tested by fecal culture. Pulsed-field gel electrophoresis analysis of isolates suggested that the strains colonizing the rectoanal junction were the same as those from the feces. Mucosal swab sampling was more sensitive than fecal sampling for determining the prevalence of E. coli O157 in feedlot cattle.

摘要

我们将粪便样本与经直肠肛门黏膜拭子（RAMS）采集的样本进行比较，以确定饲养场牛（n = 747）中大肠杆菌O157的流行情况。通过RAMS采集的样本中，9.5%检测出大肠杆菌O157，而粪便培养检测的样本中这一比例为4.7%。对分离菌株的脉冲场凝胶电泳分析表明，定殖于直肠肛门连接处的菌株与粪便中的菌株相同。对于确定饲养场牛中大肠杆菌O157的流行情况，黏膜拭子采样比粪便采样更敏感。