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对莫诺湖甲烷单加氧酶基因的分析表明,甲烷氧化活性的增加可能与甲烷营养菌群落结构的变化相关。

Analysis of methane monooxygenase genes in mono lake suggests that increased methane oxidation activity may correlate with a change in methanotroph community structure.

作者信息

Lin Ju-Ling, Joye Samantha B, Scholten Johannes C M, Schäfer Hendrik, McDonald Ian R, Murrell J Colin

机构信息

Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, England.

出版信息

Appl Environ Microbiol. 2005 Oct;71(10):6458-62. doi: 10.1128/AEM.71.10.6458-6462.2005.

Abstract

Mono Lake is an alkaline hypersaline lake that supports high methane oxidation rates. Retrieved pmoA sequences showed a broad diversity of aerobic methane oxidizers including the type I methanotrophs Methylobacter (the dominant genus), Methylomicrobium, and Methylothermus, and the type II methanotroph Methylocystis. Stratification of Mono Lake resulted in variation of aerobic methane oxidation rates with depth. Methanotroph diversity as determined by analysis of pmoA using new denaturing gradient gel electrophoresis primers suggested that variations in methane oxidation activity may correlate with changes in methanotroph community composition.

摘要

莫诺湖是一个碱性超盐湖,具有很高的甲烷氧化速率。检索到的pmoA序列显示,需氧甲烷氧化菌种类繁多,包括I型甲烷营养菌甲基杆菌属(优势属)、甲基微菌属和嗜热甲基菌属,以及II型甲烷营养菌甲基孢囊菌属。莫诺湖的分层导致需氧甲烷氧化速率随深度变化。使用新的变性梯度凝胶电泳引物通过pmoA分析确定的甲烷营养菌多样性表明,甲烷氧化活性的变化可能与甲烷营养菌群落组成的变化相关。

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