使用东曹DEAE 650 S对多种形式的红细胞酶进行高分辨率分析。

High resolution of multiple forms of red blood cell enzymes using a Toyopearl DEAE 650 S.

作者信息

Stocchi V, Masat L, Biagiarelli B, Accorsi A, Piccoli G, Palma F, Cucchiarini L, Dachà M

机构信息

Istituto di Chimica Biologica, Giorgio Fornaini, Università degli Studi, Urbino, Italy.

出版信息

Prep Biochem. 1992 Mar;22(1):11-40. doi: 10.1080/10826069208018017.

DOI:10.1080/10826069208018017

PMID:1620685

Abstract

We have investigated a new anion exchange chromatographic support (Toyopearl DEAE 650 S) which simultaneously allows easily to remove hemoglobin from hemolysates and to obtain a very high resolution of enzymes present in multiple forms. The results obtained are better than those obtainable using an anion-exchange HPLC column. The data obtained at analytical level suggest a wider use of this new matrix also for preparative purposes without significant changes in the resolution.

摘要

我们研究了一种新型阴离子交换色谱支持物（Toyopearl DEAE 650 S），它能同时轻松地从溶血产物中去除血红蛋白，并对多种形式存在的酶实现非常高的分辨率。所得结果优于使用阴离子交换HPLC柱所能获得的结果。在分析水平上获得的数据表明，这种新基质也可更广泛地用于制备目的，而分辨率不会有显著变化。