Asano Masahiro, Kubota Satoshi, Nakanishi Tohru, Nishida Takashi, Yamaai Tomoichiro, Yosimichi Gen, Ohyama Kazumi, Sugimoto Tomosada, Murayama Yoji, Takigawa Masaharu
Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Cell Commun Signal. 2005 Oct 5;3:11. doi: 10.1186/1478-811X-3-11.
CCN2/CTGF is known to be involved in tooth germ development and periodontal tissue remodeling, as well as in mesenchymal tissue development and regeneration. In this present study, we investigated the roles of CCN2/CTGF in the proliferation and differentiation of periodontal ligament cells (murine periodontal ligament-derived cell line: MPL) in vitro.
In cell cultures of MPL, the mRNA expression of the CCN2/CTGF gene was stronger in sparse cultures than in confluent ones and was significantly enhanced by TGF-beta. The addition of recombinant CCN2/CTGF (rCCN2) to MPL cultures stimulated DNA synthesis and cell growth in a dose-dependent manner. Moreover, rCCN2 addition also enhanced the mRNA expression of alkaline phosphatase (ALPase), type I collagen, and periostin, the latter of which is considered to be a specific marker of the periosteum and periodontium; whereas it showed little effect on the mRNA expression of typical osteoblastic markers, e.g., osteopontin and osteocalcin. Finally, rCCN2/CTGF also stimulated ALPase activity and collagen synthesis.
These results taken together suggest important roles of CCN2/CTGF in the development and regeneration of periodontal tissue including the periodontal ligament.
已知CCN2/结缔组织生长因子参与牙胚发育和牙周组织重塑，以及间充质组织发育和再生。在本研究中，我们调查了CCN2/结缔组织生长因子在体外对牙周膜细胞（小鼠牙周膜来源细胞系：MPL）增殖和分化的作用。
在MPL细胞培养中，CCN2/结缔组织生长因子基因的mRNA表达在稀疏培养中比汇合培养中更强，并且被转化生长因子-β显著增强。向MPL培养物中添加重组CCN2/结缔组织生长因子（rCCN2）以剂量依赖方式刺激DNA合成和细胞生长。此外，添加rCCN2还增强了碱性磷酸酶（ALPase）、I型胶原和骨膜蛋白的mRNA表达，其中骨膜蛋白被认为是骨膜和牙周膜的特异性标志物；而它对典型成骨细胞标志物如骨桥蛋白和骨钙素的mRNA表达几乎没有影响。最后，rCCN2/结缔组织生长因子还刺激了ALPase活性和胶原合成。
这些结果共同表明CCN2/结缔组织生长因子在包括牙周膜在内的牙周组织发育和再生中起重要作用。
