Jin Bo, Yin Pinghe, Ma Yihong, Zhao Ling
SA Water Centre of Water Science and Systems, University of South Australia, Mawson Lakes, SA 5095, Australia.
J Ind Microbiol Biotechnol. 2005 Dec;32(11-12):678-86. doi: 10.1007/s10295-005-0045-4. Epub 2005 Oct 6.
This study proposed a novel waste utilization bioprocess for production of lactic acid and fungal biomass from waste streams by fungal species of Rhizopus arrhizus 36017 and R. oryzae 2062. The lactic acid and fungal biomass were produced in a single-stage simultaneous saccharification and fermentation process using potato, corn, wheat and pineapple waste streams as production media. R. arrhizus 36017 gave a high lactic acid yield up to 0.94-0.97 g/g of starch or sugars associated with 4-5 g/l of fungal biomass produced, while 17-19 g/l fungal biomass with a lactic acid yield of 0.65-0.76 g/g was produced by the R. oryzae 2062 in 36-48 h fermentation. Supplementation of 2 g/l of ammonium sulfate, yeast extract and peptone stimulated an increase in 8-15% lactic acid yield and 10-20% fungal biomass.
本研究提出了一种新型的废物利用生物工艺,用于利用无根根霉36017和米根霉2062从废物流中生产乳酸和真菌生物质。乳酸和真菌生物质是在单阶段同步糖化发酵过程中产生的,使用马铃薯、玉米、小麦和菠萝废物流作为生产培养基。无根根霉36017的乳酸产量高达0.94-0.97克/克淀粉或糖,同时产生4-5克/升的真菌生物质,而米根霉2062在36-48小时的发酵中产生17-19克/升的真菌生物质,乳酸产量为0.65-0.76克/克。添加2克/升硫酸铵、酵母提取物和蛋白胨可使乳酸产量提高8-15%,真菌生物质增加10-20%。