在为期一年的研究中，腹泻患者粪便标本中存在游离志贺样毒素。

The presence of free shiga-like toxins in stool specimens of patients with diarrhea during one year study.

作者信息

Sobieszczańska Beata M, Kuzko Katarzyna, Gryko Romuald, Dobrowolska Magdalena

机构信息

University of Medicine, Department of Microbiology, 4 Chalubifiskiego Street, 50-368 Wrocław, Poland.

出版信息

Pol J Microbiol. 2005;54(1):69-71.

PMID:16209098

Abstract

During one year study, 394 stool samples obtained from a random cases of diarrhea were examined for the presence of free shiga-like toxins Stx1 and Stx2 in Vero cells assay. Of 394 stool specimen supernatants tested, 2 (0.5%) gave positive results. The two stool supernatants positive on Vero cell line were wholly capable of being neutralized with the monoclonal antibody to Stx2. Broth cultures of strains isolated from the two positive stool samples were negative for Stxs-production in Vero cytotoxicity assay and by PCR.

摘要

在为期一年的研究中，对从腹泻随机病例中获取的394份粪便样本进行检测，通过Vero细胞试验检测其中是否存在游离的志贺样毒素Stx1和Stx2。在检测的394份粪便标本上清液中，有2份（0.5%）呈阳性结果。在Vero细胞系上呈阳性的两份粪便上清液完全能够被抗Stx2单克隆抗体中和。从两份阳性粪便样本中分离出的菌株的肉汤培养物，在Vero细胞毒性试验和PCR检测中，Stxs产生均为阴性。