Kumar H S, Karunasagar Indrani, Karunasagar I, Teizou Tsukamoto, Shima Kensuke, Yamasaki Shinji
Department of Fishery Microbiology, University of Agricultural Sciences, College of Fisheries, Mangalore 575 002, India.
FEMS Microbiol Lett. 2004 Apr 1;233(1):173-8. doi: 10.1016/j.femsle.2004.02.008.
Shiga toxin-producing Escherichia coli (STEC) strains isolated in Mangalore, India, were characterised by bead-enzyme-linked immunosorbent assay (bead-ELISA), Vero cell cytotoxicity assay, PCR and colony hybridisation for the detection of stx1 and stx2 genes. Four strains from seafood, six from beef and one from a clinical case of bloody diarrhoea were positive for Shiga toxins Stx1 and Stx2 and also for stx1and stx2 genes. The seafood isolates produced either Stx2 alone or both Stx1 and Stx2, while the beef isolates produced Stx1 alone. The stx1 gene of all the beef STEC was found to be of recently reported stx1c type. All STEC strains and one non-STEC strain isolated from clam harboured EHEC-hlyA. Interestingly, though all STEC strains were negative for eae gene, two STEC strains isolated from seafood and one from a patient with bloody diarrhoea possessed STEC autoagglutinating adhesion (saa) gene, recently identified as a gene encoding a novel autoagglutinating adhesion.
对在印度芒格洛尔分离出的产志贺毒素大肠杆菌（STEC）菌株进行了研究，采用珠酶联免疫吸附测定法（珠式ELISA）、Vero细胞细胞毒性测定法、聚合酶链反应（PCR）和菌落杂交法检测stx1和stx2基因。来自海产品的4株菌株、来自牛肉的6株菌株以及来自1例血性腹泻临床病例的1株菌株，志贺毒素Stx1和Stx2以及stx1和stx2基因均呈阳性。海产品分离株单独产生Stx2或同时产生Stx1和Stx2，而牛肉分离株仅产生Stx1。所有牛肉STEC的stx1基因均为最近报道的stx1c型。从蛤中分离出的所有STEC菌株和1株非STEC菌株均携带肠出血性大肠杆菌溶血素A（EHEC-hlyA）。有趣的是，尽管所有STEC菌株的eae基因均为阴性，但从海产品中分离出的2株STEC菌株和1株来自血性腹泻患者的菌株携带STEC自凝黏附素（saa）基因，该基因最近被鉴定为编码一种新型自凝黏附素的基因。
