气单胞菌属菌株周质蛋白电泳图谱的数值分析

Numerical analysis of electrophoretic periplasmic protein patterns of Aeromonas sp. strains.

作者信息

Szczuka Ewa, Kaznowski Adam

机构信息

Department of Microbiology, Institute of Experimental Biology, Adam Mickiewicz University, ul. Fredry 10, 61-701 Poznań, Poland.

出版信息

Pol J Microbiol. 2005;54(2):91-5.

PMID:16209101

Abstract

A total of 103 strains of Aeromonas spp. isolated from clinical and from environmental samples was compared by using SDS-PAGE of periplasmic proteins patterns. Strains isolated from Polish children suffering from gastroenteritis did not appear similar to strains isolated from human living in Hong-Kong. Aeromonas sp. strains did not show a tendency to cluster according to their origin. Our results have demonstrated no species-specific periplasmic protein profiles. A significant protein electrophoretic heterogeneity was observed within the species A. hydrophila, A. bestiarum, A. salmonicida, A. caviae, A. media, and A. veronii biotype sobria.

摘要

运用周质蛋白模式的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE），对从临床和环境样本中分离出的总共103株气单胞菌属菌株进行了比较。从患有肠胃炎的波兰儿童中分离出的菌株，与从生活在香港的人群中分离出的菌株看起来并不相似。气单胞菌属菌株并未表现出根据其来源聚类的趋势。我们的结果表明不存在物种特异性的周质蛋白谱。在嗜水气单胞菌、杀鲑气单胞菌、豚鼠气单胞菌、中间气单胞菌和维氏气单胞菌温和生物型内，观察到了显著的蛋白质电泳异质性。