Krawczyk Beata, Samet Alfred, Czarniak Elzbieta, Szczapa Jerzy, Kur Józef
Department of Microbiology, Gdańsk University of Technology, ul. G. Narutowicza 11/12, 80-952 Gdańsk, Poland.
Pol J Microbiol. 2005;54(2):105-10.
Extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae (EPKP) strains are frequently implicated in outbreaks in neonatal units. From April 2002 to January 2003, 149 neonates were colonized/infected with EPKP in the Neonatal Clinic of the Teaching Hospital at the Medical University of Gdańsk, Poland. A novel assay based on suppression of PCR, ADSRRS-fingerprinting, was successfully evaluated for typing EPKP isolates. The results showed that the genotypes of all outbreak-related strains were identical, which suggested that the outbreak originated from a single clone. This conclusion was confirmed by using different methods--RAPD and PFGE. The outbreak was stopped by adopting improved hygiene and instituting outbreak control measures.
产超广谱β-内酰胺酶（ESBL）的肺炎克雷伯菌（EPKP）菌株常与新生儿病房的暴发有关。2002年4月至2003年1月，波兰格但斯克医科大学教学医院新生儿诊所的149名新生儿被EPKP定植/感染。一种基于PCR抑制的新型检测方法，即ADSRRS指纹图谱法，成功用于对EPKP分离株进行分型。结果显示，所有与暴发相关菌株的基因型相同，这表明暴发源自单一克隆。采用不同方法（随机扩增多态性DNA分析和脉冲场凝胶电泳）证实了这一结论。通过改善卫生条件和采取暴发控制措施，此次暴发得以遏制。
