Shen Guo-qiu, Lu Gong-cheng, Pan Tie-jun, Xiao Ya-jun
Department of Urology, Wuhan General Hospital of Guanzhou Command, PLA, Wuhan, Hubei 430070, China.
Zhonghua Nan Ke Xue. 2005 Sep;11(9):664-6, 669.
To evaluate the effect of the levels of IGF-I in the epididymis and the expression of IGF-I in the testis of adult male rat after the administration of cyclophosphamide.
Ninety-six male adult rats (8 weeks age) were divided into 6 groups. The doses given to the rats of the groups 1 to 5 were 10, 20, 40, 80 and 100 mg/(kg x d), respectively. The remaining group was served as control. All those rats were sacrificed and IGF-I were quantitatively determined by ELISA techniques 2 and 4 weeks after the administration of the drug (by gastric fudge). Immunohistochemical SP technique was used to examine expression of IGF-I in rat testis.
The levels of cell factors (IGF-I) in the epididymis of the rats were gradually reduced with the increasing time and dose after administration of the drug. In the mean time the expression of IGF-I in the tissues of the testis of those rats were also gradually reduced.
In the time of oligozoospermia/azoospermia induced by the administration of cyclophosphamide, the expression levels of IGF-I in the genetic system were significantly reduced. The possible mechanism of these changes could be attributed to the lower spermatogenesis function of the testis caused by the administration of cyclophosphamide.
评估环磷酰胺给药后成年雄性大鼠附睾中胰岛素样生长因子-I（IGF-I）水平及睾丸中IGF-I表达的变化。
将96只8周龄雄性成年大鼠分为6组。第1至5组大鼠的给药剂量分别为10、20、40、80和100mg/(kg·d)。其余组作为对照组。所有大鼠在给药（经胃灌服）后2周和4周处死，采用酶联免疫吸附测定（ELISA）技术定量检测IGF-I。采用免疫组织化学SP法检测大鼠睾丸中IGF-I的表达。
给药后，大鼠附睾中细胞因子（IGF-I）水平随时间和剂量增加逐渐降低。同时，这些大鼠睾丸组织中IGF-I的表达也逐渐降低。
在环磷酰胺诱导少精子症/无精子症时，遗传系统中IGF-I的表达水平显著降低。这些变化的可能机制可能是环磷酰胺给药导致睾丸生精功能降低。
