Vallejos Jose R, Kostov Yordan, Marten Mark R, Rao Govind
Department of Chemical and Biochemical Engineering, Center for Advanced Sensor Technology, University of Maryland at Baltimore County, Baltimore, Maryland 21250, USA.
Biotechnol Prog. 2005 Sep-Oct;21(5):1531-6. doi: 10.1021/bp050117x.
A novel confocal optical system to study mixing time in small-scale bioreactors is presented. The system is designed to monitor fluorescence upon tracer addition from a localized confocal volume of 0.21 mL within a glass vessel. The key elements of the fluorescence-based confocal system are a pinhole, a lens, an APD (Avalanche photodiode) detector, and light filters. The optical technique was validated by comparison with a pH-based technique. Finally, the optical sensor was tested and a real cultivation media (i.e., spent mammalian cell media) was used to measure mixing time in a 12.5-mL stirred transparent vessel. High accuracy, easy results interpretation, and low costs are the three most attractive characteristics of the sensor. Because of its noninvasive nature and versatility, the results suggest that the confocal system is a promising tool to perform mixing time studies in stirred vessels.
本文介绍了一种用于研究小型生物反应器中混合时间的新型共聚焦光学系统。该系统旨在监测从玻璃容器内0.21 mL的局部共聚焦体积中添加示踪剂后的荧光。基于荧光的共聚焦系统的关键元件是针孔、透镜、雪崩光电二极管（APD）探测器和滤光片。通过与基于pH值的技术进行比较，验证了该光学技术。最后，对光学传感器进行了测试，并使用实际的培养基（即废弃的哺乳动物细胞培养基）来测量12.5 mL搅拌透明容器中的混合时间。高精度、易于结果解读和低成本是该传感器最具吸引力的三个特点。由于其非侵入性和多功能性，结果表明共聚焦系统是在搅拌容器中进行混合时间研究的一种有前途的工具。
