用于内脏利什曼病血清诊断的重组利什曼原虫抗原。
Recombinant leishmania antigens for serodiagnosis of visceral leishmaniasis.
作者信息
Passos S, Carvalho L P, Orge G, Jerônimo S M, Bezerra G, Soto M, Alonso C, Carvalho E M
机构信息
Serviço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia, 5 andar, Rua João das Botas, s/n Canela, 40110-160, Salvador, Bahia, Brazil.
出版信息
Clin Diagn Lab Immunol. 2005 Oct;12(10):1164-7. doi: 10.1128/CDLI.12.10.1164-1167.2005.
Serological tests with crude or recombinant Leishmania antigens are important tools for the diagnosis of leishmania infection. However, these tests are not markers of active visceral leishmaniasis (VL), since antibodies to these markers are often observed in individuals with subclinical L. chagasi infection and they do not fall shortly after therapy. In this study, levels of immunoglobulin G (IgG) against three recombinant Leishmania antigens (rH2A, KMP11, and the "Q" protein) were evaluated in sera from individuals with subclinical L. chagasi infection and in patients with VL pre- and posttherapy. The sensitivity of the serological test for diagnosis of VL was 100% with all three antigens. The titers of IgG fell significantly after therapy. While most of the individuals with subclinical L. chagasi infection had antibodies to rH2A and the "Q" protein, only 1 out of 15 individuals had antibodies to KMP11. These data indicate that KMP11 may be used to discriminate L. chagasi infection from active VL and may serve as a marker of response to therapy.
使用粗制或重组利什曼原虫抗原进行血清学检测是诊断利什曼原虫感染的重要工具。然而，这些检测并非活动性内脏利什曼病（VL）的标志物，因为在亚临床恰加斯利什曼原虫感染个体中常可观察到针对这些标志物的抗体，且在治疗后这些抗体水平不会很快下降。在本研究中，评估了亚临床恰加斯利什曼原虫感染个体以及VL患者治疗前后血清中针对三种重组利什曼原虫抗原（rH2A、KMP11和“Q”蛋白）的免疫球蛋白G（IgG）水平。使用这三种抗原进行VL诊断的血清学检测敏感性均为100%。治疗后IgG滴度显著下降。虽然大多数亚临床恰加斯利什曼原虫感染个体有针对rH2A和“Q”蛋白的抗体，但15名个体中只有1人有针对KMP11的抗体。这些数据表明，KMP11可用于区分恰加斯利什曼原虫感染与活动性VL，并且可能作为治疗反应的标志物。
Zotero 插件