一种新的利什曼原虫假设蛋白可用于准确诊断犬和人内脏利什曼病，并可作为人类疾病的潜在预后标志物。
A new Leishmania hypothetical protein can be used for accurate serodiagnosis of canine and human visceral leishmaniasis and as a potential prognostic marker for human disease.
机构信息
Instituto de Ensino e Pesquisa, Santa Casa de Belo Horizonte, Rua Domingos Vieira, 590, Santa Efigênia, 30150-240, Belo Horizonte, Minas Gerais, Brazil; Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, 30130-100, Minas Gerais, Brazil.
Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, 30130-100, Minas Gerais, Brazil.
出版信息
Exp Parasitol. 2020 Sep;216:107941. doi: 10.1016/j.exppara.2020.107941. Epub 2020 Jul 2.
Distinct antigens have been evaluated with diagnostic purpose for canine and human visceral leishmaniasis (VL), and variable sensitivity and specificity values have been obtained in the assays. In the present study, a Leishmania infantum hypothetical protein called LiHyG, which was identified in an immunoproteomics study in Leishmania infantum amastigote extracts by antibodies in VL dogs sera; was cloned, expressed, purified and evaluated as a recombinant protein (rLiHyG) for the diagnosis of canine and human disease. The recombinant amastigote-specific A2 protein (rA2) and a soluble L. infantum protein extract (SLA) were used as controls. For canine VL, the sensitivity values were of 100%, 57.29% and 48.57%, when rLiHyG, rA2 and SLA were used, respectively, while the specificity values were of 100%, 81.43% and 88.57%, respectively. In addition, AUC values were of 1.00, 0.72 and 0.65, when rLiHyG, rA2 and SLA were used, respectively, while accuracy was of 100%, 72.38% and 75.24%, respectively. For human VL, the sensitivity values were of 100%, 84.00% and 88.00%, when rLiHyG, rA2 and SLA were used, respectively, while the specificity values were of 100%, 58.75% and 73.75%, respectively. In addition, AUC values were of 1.00, 0.76 and 0.83, when rLiHyG, rA2 and SLA were used, respectively, while accuracy was of 100%, 64.8% and 66.6%, respectively. The prognostic role of rLiHyG in the human VL was also evaluated, by means of post-therapeutic serological follow-up with sera samples collected before and six months after treatment. Results showed that treated patients presented significant reductions in the anti-rLiHyG IgG, IgG1, and IgG2 antibody levels, with results being similar to those found in healthy subjects. Testing the rA2 protein and SLA as antigens, lower IgG, IgG1, and IgG2 levels were also found, although they were higher after treatment than those obtained for rLiHyG. In conclusion, results suggested that rLiHyG could be considered for future studies as a diagnostic and/or prognostic marker for canine and human VL.
已经评估了用于犬内脏利什曼病（VL）和人内脏利什曼病的具有诊断目的的不同抗原，并且在检测中获得了可变的敏感性和特异性值。在本研究中，鉴定了一种利什曼原虫假设蛋白，称为 LiHyG，它是在利什曼原虫无鞭毛体提取物的免疫蛋白质组学研究中，通过 VL 狗血清中的抗体鉴定的；被克隆、表达、纯化并作为重组蛋白（rLiHyG）进行评估，用于诊断犬和人疾病。重组无鞭毛体特异性 A2 蛋白（rA2）和可溶性 L. infantum 蛋白提取物（SLA）用作对照。对于犬内脏利什曼病，当分别使用 rLiHyG、rA2 和 SLA 时，敏感性值分别为 100%、57.29%和 48.57%，而特异性值分别为 100%、81.43%和 88.57%。此外，当使用 rLiHyG、rA2 和 SLA 时，AUC 值分别为 1.00、0.72 和 0.65，而准确度分别为 100%、72.38%和 75.24%。对于人内脏利什曼病，当分别使用 rLiHyG、rA2 和 SLA 时，敏感性值分别为 100%、84.00%和 88.00%，而特异性值分别为 100%、58.75%和 73.75%。此外，当使用 rLiHyG、rA2 和 SLA 时，AUC 值分别为 1.00、0.76 和 0.83，而准确度分别为 100%、64.8%和 66.6%。还通过在治疗前后收集的血清样本进行治疗后血清学随访，评估了 rLiHyG 在人内脏利什曼病中的预后作用。结果表明，治疗患者的抗-rLiHyG IgG、IgG1 和 IgG2 抗体水平显著降低，结果与健康受试者相似。检测 rA2 蛋白和 SLA 作为抗原时，也发现 IgG、IgG1 和 IgG2 水平较低，但治疗后高于 rLiHyG 获得的水平。总之，结果表明 rLiHyG 可考虑作为犬和人内脏利什曼病的诊断和/或预后标志物进行进一步研究。
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