用于测量抗肺炎链球菌功能性抗体的荧光多价调理吞噬试验。
Fluorescent multivalent opsonophagocytic assay for measurement of functional antibodies to Streptococcus pneumoniae.
作者信息
Bieging Kathryn T, Rajam Gowrisankar, Holder Patricia, Udoff Ross, Carlone George M, Romero-Steiner Sandra
机构信息
Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA.
出版信息
Clin Diagn Lab Immunol. 2005 Oct;12(10):1238-42. doi: 10.1128/CDLI.12.10.1238-1242.2005.
Abstract
We developed fluorescent mono- and multivalent opsonophagocytic assays (fOPA and fmOPA, respectively) specific for seven Streptococcus pneumoniae serotypes (4, 6B, 9V, 14, 18C, 19F, and 23F). Bacterial survival was quantitated with alamar blue, a fluorescent metabolic indicator. Both fOPA and fmOPA allow for determination of viability endpoints for up to seven serotypes with high levels of agreement to the reference method. The fmOPA eliminates colony counting, reduces serum volume, and produces results in 1 day.
摘要
我们开发了分别针对七种肺炎链球菌血清型（4、6B、9V、14、18C、19F和23F）的荧光单价和多价调理吞噬试验（分别为fOPA和fmOPA）。用荧光代谢指示剂alamar blue对细菌存活率进行定量。fOPA和fmOPA都能确定多达七种血清型的活力终点，与参考方法的一致性很高。fmOPA无需进行菌落计数，减少了血清用量，并在1天内得出结果。
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