Harris Melanie J, Boddy Lynne
Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3TL, UK.
Microb Ecol. 2005 Aug;50(2):141-51. doi: 10.1007/s00248-004-0075-x. Epub 2005 Oct 13.
The effect of arrival of wood resources, pre-colonized by Coriolus versicolor, Phlebia radiata, Stereum hirsutum, and Vuilleminia comedens, on mycelial systems of Phanerochaete velutina was studied in trays of nonsterile soil in the laboratory over 5 months. Morphological responses were quantified non-destructively using image analysis. In a parallel series of experiments, nutrient movement was also quantified non-destructively using (32)P monitoring with a scintillation probe and subsequently by destructively harvesting after 155 days. The presence of a fungus occupying a newly arriving resource had major effects on deployment of biomass and on the uptake and allocation of phosphorus in the established Pha. velutina system. The effects varied depending on the species occupying the new resource. Hyphal coverage was greater in the half of the system to which new resources were added. Following addition of new resources, there was massive redeployment of biomass away from regions with no new resource when the new resource was (1) uncolonized, (2) colonized by V. comedens, or (3) colonized by S. hirsutum (although to a lesser extent with the latter), but not with others. (32)P was taken up by Pha. velutina both in the vicinity of the inoculum and the new resource and was translocated to the new resource from both sites of uptake; however, the local supply contributed most. Bidirectional translocation also occurred. The results are discussed in relation to mycelial foraging strategies, nutrient translocation, and partitioning within mycelial cord systems.
在实验室的非无菌土壤培养皿中，对由云芝、辐射状脉菌、毛栓菌和小孢拟层孔菌预先定殖的木材资源到达后，对绒毛栓菌菌丝系统的影响进行了为期5个月的研究。使用图像分析对形态学反应进行无损量化。在一系列平行实验中，还使用闪烁探头通过³²P监测对养分移动进行无损量化，随后在155天后通过破坏性收获进行量化。一种真菌占据新到达的资源对已建立的绒毛栓菌系统中生物量的分布以及磷的吸收和分配有重大影响。影响因占据新资源的物种而异。在添加新资源的系统一半中，菌丝覆盖率更高。添加新资源后，当新资源为（1）未被定殖、（2）被小孢拟层孔菌定殖或（3）被毛栓菌定殖（尽管后者程度较小）时，生物量会从没有新资源的区域大量重新分布，但其他情况则不会。³²P在接种物附近和新资源处均被绒毛栓菌吸收，并从两个吸收位点转运至新资源；然而，局部供应贡献最大。双向转运也会发生。结合菌丝觅食策略、养分转运以及菌丝索系统内的分配对结果进行了讨论。
