Couté Yohann, Burgess Jennifer A, Diaz Jean-Jacques, Chichester Christine, Lisacek Frédérique, Greco Anna, Sanchez Jean-Charles
Biomedical Proteomics Research Group, Département de Biologie Structurale et Bioinformatique, Centre Médical Universitaire, 1 Rue Michel Servet, 1211 Geneva 14, Switzerland.
Mass Spectrom Rev. 2006 Mar-Apr;25(2):215-34. doi: 10.1002/mas.20067.
Nucleoli are plurifunctional nuclear domains involved in the regulation of several major cellular processes such as ribosome biogenesis, the biogenesis of non-ribosomal ribonucleoprotein complexes, cell cycle, and cellular aging. Until recently, the protein content of nucleoli was poorly described. Several proteomic analyses have been undertaken to discover the molecular bases of the biological roles fulfilled by nucleoli. These studies have led to the identification of more than 700 proteins. Extensive bibliographic and bioinformatic analyses allowed the classification of the identified proteins into functional groups and suggested potential functions of 150 human proteins previously uncharacterized. The combination of improvements in mass spectrometry technologies, the characterization of protein complexes, and data mining will assist in furthering our understanding of the role of nucleoli in different physiological and pathological cell states.
核仁是多功能的核结构域，参与多种主要细胞过程的调控，如核糖体生物合成、非核糖体核糖核蛋白复合物的生物合成、细胞周期和细胞衰老。直到最近，核仁的蛋白质组成仍鲜有描述。已经进行了多项蛋白质组学分析，以发现核仁所发挥生物学作用的分子基础。这些研究已鉴定出700多种蛋白质。广泛的文献和生物信息学分析使所鉴定的蛋白质能够被分类为功能组，并提示了150种以前未被表征的人类蛋白质的潜在功能。质谱技术的改进、蛋白质复合物的表征和数据挖掘的结合，将有助于加深我们对核仁在不同生理和病理细胞状态中作用的理解。
