Yamada Elizabeth S, Bordt Andrea S, Marshak David W
Department of Neurobiology and Anatomy, University of Texas Medical School, Houston, TX 77225, USA.
Vis Neurosci. 2005 Jul-Aug;22(4):383-93. doi: 10.1017/S095252380522401X.
To describe the wide-field ganglion cells, they were injected intracellularly with Neurobiotin using an in vitro preparation of macaque retina and labeled with streptavidin-Cy3. The retinas were then labeled with antibodies to choline acetyltransferase and other markers to indicate the depth of the dendrites within the inner plexiform layer (IPL) and analyzed by confocal microscopy. There were eight different subtypes of narrowly unistratified cells that ramified in each of the 5 strata, S1-5, including narrow thorny, large sparse, large moderate, large dense, large radiate, narrow wavy, large very sparse, and fine very sparse. There were four types of broadly stratified cells with dendritic trees extending from S4 to S2. One type resembled the parvocellular giant cell and another the broad thorny type described previously in primates. Another broadly stratified cell was called multi-tufted based on its distinctive dendritic branching pattern. The fourth type had been described previously, but not named; we called it broad wavy. There was a bistratified type with its major arbor in S5, the same level as the blue cone bipolar cell; it resembled the large, bistratified cell with blue ON-yellow OFF responses described recently. Two wide-field ganglion cell types were classified as diffuse because they had dendrites throughout the IPL. One had many small branches and was named thorny diffuse. The second was named smooth diffuse because it had straighter dendrites that lacked these processes. Dendrites of the large moderate and multi-tufted cells cofasciculated with ON-starburst cell dendrites and were, therefore, candidates to be ON- and ON-OFF direction-selective ganglion cells, respectively. We concluded that there are at least 15 morphoplogical types of wide-field ganglion cells in macaque retinas.
为了描述广域神经节细胞，我们在猕猴视网膜的体外制备物中对其进行细胞内注射神经生物素并用链霉亲和素 - Cy3标记。然后用抗胆碱乙酰转移酶抗体和其他标记物对视网膜进行标记，以指示内网状层（IPL）内树突的深度，并通过共聚焦显微镜进行分析。有8种不同亚型的窄单分层细胞，它们在S1 - 5这5个分层中的每一层都有分支，包括窄棘状、大稀疏、大中等、大密集、大辐射状、窄波浪状、大非常稀疏和细非常稀疏。有4种宽分层细胞，其树突从S4延伸到S2。一种类似于小细胞巨细胞，另一种类似于先前在灵长类动物中描述的宽棘状类型。另一种宽分层细胞因其独特的树突分支模式而被称为多簇状。第四种类型先前已有描述，但未命名；我们将其称为宽波浪状。有一种双分层类型，其主要分支在S5，与蓝锥双极细胞处于同一水平；它类似于最近描述的具有蓝ON - 黄OFF反应的大双分层细胞。两种广域神经节细胞类型被归类为弥散型，因为它们的树突遍布整个IPL。一种有许多小分支，被命名为棘状弥散型。第二种被命名为平滑弥散型，因为它有更直的树突且没有这些分支。大中等和多簇状细胞的树突与ON - 星爆细胞的树突共束状排列，因此分别有可能是ON和ON - OFF方向选择性神经节细胞。我们得出结论，猕猴视网膜中至少有15种形态类型的广域神经节细胞。
