Wu Qingli, Feng Yonghong, Yang Yifu, Zhou Wenliang, He Peilan, Zhou Ru, Li Xiaoyu, Zou Jianping
Laboratory of Immunopharmacology & State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China.
Cell Mol Immunol. 2004 Feb;1(1):57-62.
This study was undertaken to have a better understanding for the process and the underlying mechanisms to limit macrophage activation and population of activated macrophages. A comprehensive kinetics of cytokine production was performed in murine peritoneal macrophages recovered from Balb/c mice at various time during the course of an intraperitoneal injection with thioglycollate (TG). The expression of cell surface molecules such as MHC-I, MHC-II, B7-1 and B7-2 of these macrophages were also determined by flow cytometry. The present findings of our research suggested that the population of activated macrophages and the activation of macrophages (including cytokines production and expression of cell surface functional molecules) were strictly controlled during inflammation process. This is one of the important mechanisms to retain the host homeostasis.
本研究旨在更好地理解限制巨噬细胞活化及活化巨噬细胞数量的过程和潜在机制。在用巯基乙酸盐（TG）进行腹腔注射的过程中，于不同时间点从Balb/c小鼠中回收鼠腹膜巨噬细胞，对细胞因子产生进行了全面的动力学研究。这些巨噬细胞的细胞表面分子如MHC-I、MHC-II、B7-1和B7-2的表达也通过流式细胞术进行了测定。我们研究的当前发现表明，在炎症过程中，活化巨噬细胞的数量以及巨噬细胞的活化（包括细胞因子产生和细胞表面功能分子的表达）受到严格控制。这是维持宿主稳态的重要机制之一。
