Baker Andy
School of Geography, Earth and Environmental Sciences, The University of Birmingham, Birmingham, B15 2TT, UK.
Water Res. 2005 Nov;39(18):4405-12. doi: 10.1016/j.watres.2005.08.023. Epub 2005 Oct 6.
The fluorescence excitation-emission matrices of dissolved organic matter (DOM) are investigated between 10 and 45 degrees C for river and waste waters and organic matter standards. With increased temperature, fluorescence intensity is quenched. It is demonstrated that for a range of river and wastewater samples, that tryptophan-like fluorescence exhibits a greater range of quenching (between 20+/-4% and 35+/-5%) than fulvic-like fluorescence (19+/-4 to 26+/-3%) over this temperature range. Humic substance standards exhibit similar fulvic-like (23+/-4%) fluorescence thermal quenching properties to river water samples (23+/-3%); however none of the samples exhibit quenching of tryptophan-like fluorescence to the same extent as the tryptophan standards (approximately 50%). Thermal fluorescence quenching is related to the exposure of the fluorophores to the heat source; our findings suggest that the tryptophan-like groups within DOM is more exposed in untreated wastewaters than in treated wastewaters riverine DOM. Thermal fluorescence properties have the potential to be used to source DOM, to provide additional chemical structural information, to temperature correct laser-induced remotely sensed DOM fluorescence, and to characterise DOM through the wastewater treatment process.
研究了河水、废水和有机物标准样品中溶解有机物（DOM）在10至45摄氏度之间的荧光激发-发射矩阵。随着温度升高，荧光强度会猝灭。结果表明，在该温度范围内，对于一系列河水和废水样品，类色氨酸荧光的猝灭范围（20±4%至35±5%）比类富里酸荧光（19±4%至26±3%）更大。腐殖质标准样品表现出与河水样品相似的类富里酸荧光热猝灭特性（23±4%）；然而，没有一个样品的类色氨酸荧光猝灭程度与色氨酸标准样品相同（约50%）。热荧光猝灭与荧光团受热源影响有关；我们的研究结果表明，DOM中的类色氨酸基团在未经处理的废水中比在处理后的废水和河流DOM中暴露得更多。热荧光特性有潜力用于确定DOM来源、提供额外的化学结构信息、对激光诱导的遥感DOM荧光进行温度校正以及通过废水处理过程对DOM进行表征。
