一种新型铜（II）三联吡啶配合物介导的氧化性DNA切割：晶体结构与DNA结合研究
Oxidative DNA cleavage mediated by a new copper (II) terpyridine complex: crystal structure and DNA binding studies.
作者信息
Uma V, Kanthimathi M, Weyhermuller T, Nair Balachandran Unni
机构信息
Chemical Laboratory, Central Leather Research Institute, Adyar, Chennai 600 020, Tamil Nadu, India.
出版信息
J Inorg Biochem. 2005 Dec;99(12):2299-307. doi: 10.1016/j.jinorgbio.2005.08.011. Epub 2005 Oct 6.
The copper (II) complex Cu(Itpy)(2)(2) (1), (Itpy=imidazole terpyridine) has been synthesized and structurally characterized. Crystal structure of the complex shows the complex to be a monomeric copper (II) species with two Itpy ligands coordinated to the metal ion to give a six coordinate complex. The complex has a distorted octahedral geometry with axial elongation. Variable temperature crystal structure data shows dynamic nature of the Jahn-Teller distortion. The complex is an avid DNA binder with a binding constant of 4.26+/-0.20x10(3)M(-1). Observed changes in the viscosity and circular dichroic spectrum of calf thymus DNA solution in the presence of complex 1 suggests intercalative binding of complex 1 to DNA. The complex cleaves supercoiled pBR322 DNA oxidatively in the presence of hydrogen peroxide.
已合成并对铜（II）配合物Cu(Itpy)(2)(2)（1）（Itpy = 咪唑三联吡啶）进行了结构表征。该配合物的晶体结构表明其为单体铜（II）物种，有两个Itpy配体与金属离子配位形成六配位配合物。该配合物具有扭曲的八面体几何结构且轴向拉长。变温晶体结构数据显示了 Jahn-Teller 畸变的动态性质。该配合物是一种 avid DNA 结合剂，结合常数为 4.26±0.20x10(3)M(-1)。在配合物 1 存在下，小牛胸腺 DNA 溶液的粘度和圆二色光谱的观察变化表明配合物 1 与 DNA 存在插入结合。该配合物在过氧化氢存在下能氧化切割超螺旋 pBR322 DNA。
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