Takahashi Kyoko, Nasu Kozue, Mashino Tadahiko, Morisaki Masuo, Hara Noriyuki, Fujimoto Yoshinori
Kyoritsu University of Pharmacy, Shibakoen, Tokyo 105-8512, Japan.
Bioorg Med Chem. 2006 Feb 1;14(3):732-8. doi: 10.1016/j.bmc.2005.08.061. Epub 2005 Oct 6.
Feeding of chemically synthesized [27-13C]codisterol ([27-13C]2), [27-13C]24-epicodisterol ([27-13C]3), [23,24-2H2]codisterol ([23,24-2H2]2), and [26,27-2H6]24-methyldesmosterol ([26,27-2H6]8) to Oryza sativa cell cultures, followed by MS and NMR analysis of the biosynthesized dihydrobrassicasterol (9)/campesterol (10), revealed that both (24R)- and (24S)-epimers of 24-methyl-Delta25-cholesterol (2/3) were converted to 9 and 10 via the common intermediate 24-methyldesmosterol (8).
将化学合成的[27-¹³C]谷甾烷醇（[27-¹³C]2）、[27-¹³C]24-表谷甾烷醇（[27-¹³C]3）、[23,24-²H₂]谷甾烷醇（[23,24-²H₂]2）和[26,27-²H₆]24-甲基胆甾烷醇（[26,27-²H₆]8）喂食给水稻细胞培养物，随后对生物合成的二氢油菜甾醇（9）/菜油甾醇（10）进行质谱和核磁共振分析，结果表明24-甲基-Δ²⁵-胆固醇（2/3）的（24R）-和（24S）-差向异构体均通过共同中间体24-甲基胆甾烷醇（8）转化为9和10。
