Almodin Carlos Gilberto, Moron Antonio Fernandes, Kulay Luiz, Minguetti-Câmara Vânia Cibele, Moraes Andréa Cristina, Torloni Maria Regina
Materbaby--Reprodução Humana e Genética, Maringá, Paraná, Brazil.
Fertil Steril. 2005 Oct;84(4):895-9. doi: 10.1016/j.fertnstert.2005.02.051.
To develop a bovine protocol for training in preimplantation genetic diagnosis (PGD) using PCR.
Randomized study.
Human reproduction PCR laboratory.
PATIENT(S): Cow ovaries obtained from slaughterhouses.
INTERVENTION(S): The ovaries were punctured and the oocytes were matured and submitted to in vitro fertilization. On the third day after fertilization, the embryos were biopsied and 1-2 blastomeres removed. A blastomere and the rest of the embryo were submitted to PCR for sex determination.
MAIN OUTCOME MEASURE(S): Establishment of a possible training protocol.
RESULT(S): A total of 50 embryos and 50 biopsied blastomeres were submitted to DNA amplification for sexing. Of the 50 embryos, 41 (82%) achieved successful DNA amplification and 9 (18%) did not. Of the 50 biopsies, 31 (62%) amplified and 19 (38%) did not. In 27 (65.9%) of the 41 embryos with DNA amplification, sex was identified as female and in 14 (34.1%) as male. In 40 cases (80%) amplification and sex determination were successful in both embryos and blastomeres. Sex was identical in all these cases.
CONCLUSION(S): This training model seems to be useful in identifying mistakes and difficulties and improving the professional's performance in the various stages of preimplantation genetic diagnosis.
