Hüttmann Kerstin, Yilmazer-Hanke Deniz, Seifert Gerald, Schramm Johannes, Pape Hans-Christian, Steinhäuser Christian
Experimental Neurobiology, Neurosurgery, University of Bonn, Sigmund Freud Str. 25, D-53105 Bonn, Germany.
Mol Cell Neurosci. 2006 Feb;31(2):210-7. doi: 10.1016/j.mcn.2005.09.011. Epub 2005 Oct 7.
Neuronal properties were investigated through patch-clamp recording in situ in surgical specimens of the human lateral amygdala (LA) obtained from patients with intractable temporal lobe epilepsy. Projection neurons displayed spiny dendrites, action potentials with varying degree of frequency adaptation, and an inwardly rectifying K+ (Kir) conductance coupled to GABA(B) receptors. In interneurons, dendrites were spineless or sparsely spiny, action potentials were shorter than those in projection neurons and often occurred spontaneously, and GABA(B) receptor-mediated responses were lacking. Single-cell RT-PCR demonstrated expression of Kir channel subunits Kir3.1 and Kir3.2 and of vesicular glutamate transporters VGLUT1 and VGLUT2 in projection neurons. It is concluded that projection neurons and interneurons of the human LA can be distinguished based upon morphological, electrophysiological, and molecular biological criteria. The most striking difference relates to the expression of postsynaptic GABA(B) receptors coupled to Kir3 channels in projection neurons and the lack of functional GABA(B) receptors in interneurons.
通过膜片钳记录法，对从难治性颞叶癫痫患者获取的人类外侧杏仁核（LA）手术标本进行原位研究，以探究神经元特性。投射神经元表现出有棘树突、具有不同程度频率适应性的动作电位，以及与GABA(B)受体偶联的内向整流钾离子（Kir）电导。在中间神经元中，树突无棘或棘少，动作电位比投射神经元的短且常自发出现，并且缺乏GABA(B)受体介导的反应。单细胞逆转录聚合酶链反应（RT-PCR）证明投射神经元中存在Kir通道亚基Kir3.1和Kir3.2以及囊泡谷氨酸转运体VGLUT1和VGLUT2的表达。得出的结论是，人类LA的投射神经元和中间神经元可根据形态学、电生理学和分子生物学标准加以区分。最显著的差异在于投射神经元中与Kir3通道偶联的突触后GABA(B)受体的表达，以及中间神经元中功能性GABA(B)受体的缺失。
