DeSalle Rob, Egan Mary G, Siddall Mark
Division of Invertebrate Zoology, American Museum of Natural History, 79th Street at Central Park West, New York, NY 10024, USA.
Philos Trans R Soc Lond B Biol Sci. 2005 Oct 29;360(1462):1905-16. doi: 10.1098/rstb.2005.1722.
Recent excitement over the development of an initiative to generate DNA sequences for all named species on the planet has in our opinion generated two major areas of contention as to how this 'DNA barcoding' initiative should proceed. It is critical that these two issues are clarified and resolved, before the use of DNA as a tool for taxonomy and species delimitation can be universalized. The first issue concerns how DNA data are to be used in the context of this initiative; this is the DNA barcode reader problem (or barcoder problem). Currently, many of the published studies under this initiative have used tree building methods and more precisely distance approaches to the construction of the trees that are used to place certain DNA sequences into a taxonomic context. The second problem involves the reaction of the taxonomic community to the directives of the 'DNA barcoding' initiative. This issue is extremely important in that the classical taxonomic approach and the DNA approach will need to be reconciled in order for the 'DNA barcoding' initiative to proceed with any kind of community acceptance. In fact, we feel that DNA barcoding is a misnomer. Our preference is for the title of the London meetings--Barcoding Life. In this paper we discuss these two concerns generated around the DNA barcoding initiative and attempt to present a phylogenetic systematic framework for an improved barcoder as well as a taxonomic framework for interweaving classical taxonomy with the goals of 'DNA barcoding'.
最近，一项为地球上所有已命名物种生成DNA序列的计划引发了人们的热议。在我们看来，关于这项“DNA条形码”计划应如何推进，产生了两个主要的争议点。在将DNA作为分类学和物种界定工具加以普遍应用之前，必须澄清并解决这两个问题。第一个问题涉及在该计划背景下如何使用DNA数据，这就是DNA条形码读取器问题（或条形码生成器问题）。目前，该计划下许多已发表的研究都采用了建树方法，更确切地说是距离法来构建用于将某些DNA序列置于分类学背景中的树。第二个问题涉及分类学界对“DNA条形码”计划指令的反应。这个问题极其重要，因为为使“DNA条形码”计划获得任何形式的学界认可，经典分类学方法和DNA方法需要相互协调。事实上，我们认为“DNA条形码”这个名称并不恰当。我们更倾向于采用伦敦会议的名称——“生命条形码”。在本文中，我们讨论围绕DNA条形码计划产生的这两个问题，并尝试提出一个用于改进条形码生成器的系统发育系统框架，以及一个将经典分类学与“DNA条形码”目标相结合的分类学框架。
