Chang M X, Nie P, Xie H X, Wang G L, Gao Y
State Key Laboratory of Freshwater Ecology and Biotechnology, and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province 430072, PR China.
Vet Immunol Immunopathol. 2006 Mar 15;110(1-2):51-63. doi: 10.1016/j.vetimm.2005.09.003. Epub 2005 Oct 7.
TRAIL (Apo2 ligand) described as a type II transmembrane protein belonging to the TNF superfamily can induce apoptotic cell death in a variety of cell types. In the present study, a putative cDNA sequence encoding the 299 amino acids of TRAIL (GC-TRAIL) and its genomic organization were identified in grass carp Ctenopharyngodon idella. The predicted GC-TRAIL sequence showed 44 and 41% identities to chicken and human TRAILs, respectively. In a domain search, a tumor necrosis factor homology domain (THD) was identified in the C-terminal portion of TRAILs. The GC-TRAIL gene consists of five exons, with four intervening introns, spaced over approximately 4 kb of genomic sequence. Analysis of GC-TRAIL promoter region revealed the presence of a number of putative transcription factor binding sites, such as Sp1, NF-kappaB, AP-1, GATA, NFAT, HNF, STAT, P53 and IRF1 sequences which are important for the expression of other TNF family members. Phylogenetic analysis placed GC-TRAIL and the putative zebrafish (Danio rerio) TRAIL obtained from searching the zebrafish database into one separate cluster near mammalian TRAIL genes, but apart from the reported zebrafish TRAIL-like protein, indicating that the GC-TRAIL is an authentic fish TRAIL. Expression analysis revealed that GC-TRAIL is expressed in many tissues, such as in gills, liver, trunk kidney, head kidney, intestine and spleen.
肿瘤坏死因子相关凋亡诱导配体（TRAIL，Apo2配体）被描述为属于肿瘤坏死因子超家族的II型跨膜蛋白，可诱导多种细胞类型发生凋亡性细胞死亡。在本研究中，在草鱼（Ctenopharyngodon idella）中鉴定出了一个推定的编码299个氨基酸的TRAIL（GC-TRAIL）的cDNA序列及其基因组结构。预测的GC-TRAIL序列与鸡和人TRAIL的同一性分别为44%和41%。在结构域搜索中，在TRAIL的C末端部分鉴定出一个肿瘤坏死因子同源结构域（THD）。GC-TRAIL基因由五个外显子组成，有四个间隔内含子，分布在约4 kb的基因组序列上。对GC-TRAIL启动子区域的分析揭示了存在许多推定的转录因子结合位点，如Sp1、NF-κB、AP-1、GATA、NFAT、HNF、STAT、P53和IRF1序列，这些对其他肿瘤坏死因子家族成员的表达很重要。系统发育分析将GC-TRAIL和从斑马鱼数据库搜索获得的推定斑马鱼（Danio rerio）TRAIL置于靠近哺乳动物TRAIL基因的一个单独聚类中，但与报道的斑马鱼类TRAIL样蛋白不同，这表明GC-TRAIL是一种真正的鱼类TRAIL。表达分析表明，GC-TRAIL在许多组织中表达，如鳃、肝脏、躯干肾、头肾、肠道和脾脏。
