Vail Timothy L, Cushing Kevin W, Ingram Jani C, St Omer Ingrid
Department of Chemistry and Biochemistry, P.O. Box 5698, Flagstaff, AZ 86011-5698, USA.
Biotechnol Appl Biochem. 2006 Feb;43(Pt 2):85-91. doi: 10.1042/BA20050085.
Processes for micropatterning protein arrays on inorganic substrates have gained attention in the development of biosensors and clinical diagnostics. This study demonstrates a chemically selective method based on photolithographic deposition of gold patterns with the subsequent attachment of functionalized alkanethiols via molecular self-assembly. Selective capping of carboxy groups on alkanethiols by N-hydroxysuccinimide esters allowed the deposition of methoxypoly(ethylene glycol) silane as a blocking agent on unpatterned regions. Carboxylates were used to couple a form of avidin to create a microarray of protein. This microarray was successfully probed with biotinylated quantum dots. In-process characterization methods included grazing-angle Fourier-transform IR spectroscopy, ellipsometry, contact-angle goniometry, atomic-force microscopy and fluorescence microscopy.
