Niittymäki Jaana, Mattila Pirkko, Renkonen Risto
Rational Drug Design program, Department of Bacteriology and Immunology, Haartman Institute and Biomedicum, University of Helsinki, Helsinki, Finland.
APMIS. 2005 Sep;113(9):613-20. doi: 10.1111/j.1600-0463.2005.apm_279.x.
The sialyl Lewis x (NeuAcalpha2-3Galbeta1-4(Fucalpha1-3)GlcNAc) determinants serve as ligands in the selectin-mediated adhesion of leukocytes to activated endothelium. The final step in the sialyl Lewis x synthesis is catalyzed by alpha1-3-fucosyltransferase, which transfers fucose to sialylated type 2 chain. We report the cloning of rat alpha1-3-fucosyltransferase gene (rFUT) isolated from rat lymph node and kidney allograft. The rFUT is expressed as two splice variants, but only the long one showed enzymatic activity towards sialylated lactosamine. Also flow cytometry analysis with the sLex mAbs indicated that the cloned rFuc-T was a functional enzyme and a member of the Fuc-TVII family. The rFuc-TVII mRNA expression level was strongly enhanced during acute inflammatory reaction induced by kidney allograft rejection, which could be detected by in situ hybridization and quantitative real-time PCR.
唾液酸化路易斯x（NeuAcalpha2-3Galbeta1-4（Fucalpha1-3）GlcNAc）决定簇作为配体，参与选择素介导的白细胞与活化内皮细胞的黏附过程。唾液酸化路易斯x合成的最后一步由α1-3-岩藻糖基转移酶催化，该酶将岩藻糖转移至唾液酸化的2型链上。我们报道了从大鼠淋巴结和肾移植组织中分离出的大鼠α1-3-岩藻糖基转移酶基因（rFUT）的克隆情况。rFUT以两种剪接变体形式表达，但只有较长的变体对唾液酸化乳糖胺表现出酶活性。此外，用sLex单克隆抗体进行的流式细胞术分析表明，克隆的rFuc-T是一种功能性酶，属于Fuc-TVII家族成员。在肾移植排斥诱导的急性炎症反应过程中，rFuc-TVII mRNA表达水平显著增强，可通过原位杂交和定量实时PCR检测到。
