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A specific and inexpensive assay of radiolabeled long-chain acyl-coenzyme A in isolated hepatocytes.

作者信息

Stals H K, Declercq P E

机构信息

Katholieke Universiteit Leuven, Instituut voor Farmaceutische Wetenschappen, Belgium.

出版信息

Anal Biochem. 1992 Apr;202(1):117-9. doi: 10.1016/0003-2697(92)90216-t.

DOI:10.1016/0003-2697(92)90216-t
PMID:1621973
Abstract

A simple procedure for determining radiolabeled long-chain acyl-CoA levels in isolated rat hepatocytes has been developed. It consists of a classical extraction of long-chain acyl-CoAs after preliminary removal of the excess labeled free fatty acid. A two-step TLC purification ensures elimination of long-chain acylcarnitine, the main interferent in most methods, and other common lipids. The purity of the acyl-CoA was confirmed by a second TLC system and by spectral analysis. Overall recovery was approximately 70%.

摘要

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