Watts Phillip C, Hamilton J Gordon C, Ward Richard D, Noyes Harry A, Souza Nataly A, Kemp Stephen J, Feliciangeli M Dora, Brazil Reginaldo, Maingon Rhayza D C
School of Biological Sciences, Liverpool University, Liverpool, United Kingdom.
Am J Trop Med Hyg. 2005 Oct;73(4):734-43.
Lutzomyia longipalpis, a sibling complex, is the main vector of Leishmania chagasi/infantum. Discriminating between siblings is important as they may differ in vectorial capacity. Lutzomyia longipalpis populations display distinct male sex pheromone chemotypes. We investigated the phylogeographic pattern of variation at microsatellite loci from 11 populations from Brazil and Venezuela related to their male pheromone. Temporal genetic differentiation was mostly not significant at the same site. Spatial genetic differentiation was, however, strong, although there was only a weak relationship between genetic differentiation and the geographic distance separating the samples (r2 < 0.10); geographic separation explained a much greater (54-97%) percentage of the genetic differences among populations when samples with the same pheromone type were analyzed separately. A cluster analysis showed five groups: Lu. cruzi (Brazil) and Lu. pseudolongipalpis (Venezuela) as separate species, two (mostly 9-methyl-germacrene-B) Venezuelan and Brazilian groups, and a very distinct cluster of Brazilian cembrene populations.
长须罗蛉是一个复合种，是恰加斯利什曼原虫/婴儿利什曼原虫的主要传播媒介。区分复合种中的各个种很重要，因为它们的传播能力可能不同。长须罗蛉种群表现出不同的雄性性信息素化学型。我们研究了来自巴西和委内瑞拉的11个种群在微卫星位点上与雄性信息素相关的系统地理变异模式。在同一地点，时间上的遗传分化大多不显著。然而，空间遗传分化很强，尽管遗传分化与样本间的地理距离之间只有微弱的关系（r2 < 0.10）；当分别分析具有相同信息素类型的样本时，地理隔离解释了种群间遗传差异的更大比例（54 - 97%）。聚类分析显示有五组：克鲁兹罗蛉（巴西）和拟长须罗蛉（委内瑞拉）为独立的物种，两组（主要是9 - 甲基 - 大根香叶烯 - B）委内瑞拉和巴西种群，以及一个非常独特的巴西桧烯种群聚类。
