Niu Cheng-Gang, Gui Xiao-Qin, Zeng Guang-Ming, Yuan Xing-Zhong
College of Environmental Science and Engineering, State Key Laboratory for Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, P. R. China.
Analyst. 2005 Nov;130(11):1551-6. doi: 10.1039/b504882f. Epub 2005 Oct 3.
This paper describes a novel ratiometric fluorescence sensor for pH measurement. Two pH-sensitive fluorophores, N-allyl-4-(4'-methyl-piperazinyl)-1,8-naphthalimide (AMPN) and meso-5,10,15,20-tetra-(4-allyloxyphenyl)porphyrin (TAPP), which served as referencing indicators for each other, were co-polymerized with acrylamide, hydroxyethyl methacrylate and triethylene glycol dimethacrylate on the silanized glass surface. The proposed sensor is based on the pH-dependent fluorescence intensities of the two fluorophores in different pH ranges. The sensor covers a broad dynamic range of pH 1.5-9.0. It exhibits satisfactory analytical performance in terms of selectivity, reproducibility and stability. The successful fabrication of the proposed sensor provides an alternative concept to utilizing two or more fluorophores for the development of ratiometric sensors covering a broad range of pH.
本文描述了一种用于pH测量的新型比率荧光传感器。两种对pH敏感的荧光团，N-烯丙基-4-(4'-甲基-哌嗪基)-1,8-萘二甲酰亚胺(AMPN)和中-5,10,15,20-四-(4-烯丙氧基苯基)卟啉(TAPP)，它们相互作为参考指示剂，与丙烯酰胺、甲基丙烯酸羟乙酯和三乙二醇二甲基丙烯酸酯在硅烷化玻璃表面上共聚。所提出的传感器基于两种荧光团在不同pH范围内依赖于pH的荧光强度。该传感器覆盖了1.5 - 9.0的宽动态pH范围。它在选择性、重现性和稳定性方面表现出令人满意的分析性能。所提出传感器的成功制备为利用两种或更多种荧光团开发覆盖宽pH范围的比率传感器提供了一种替代概念。
