Zhao Jin, Liu Xiao-Ju
Medical College of Northwest Minorities University, Lanzhou, People's Republic of China.
Pharmazie. 2005 Sep;60(9):683-8.
We have previously reported on the DNA oxidative damage and oxygen stress in healthy term neonates. The aim of the present study was to investigate the antioxidative and immunomodulatory role of melatonin, sodium selenite, N-acetyl-L-cysteine and quercetin on umbilical blood. The single cell gel electrophoresis (comet) assay was used for DNA oxidative damage. The lymphocytes proliferation and natural killer (NK) activity in umbilical blood were assayed by 3[H]-thymidine uptaken and MTT methods. Results using comet assay showed protection by melatonin, N-acetyl-L-cysteine (NAC) and quercetin, and a protective and damaging effect by selenite sodium. Melatonin, sodium selenite, NAC and quercetin greatly promoted the lymphocytes proliferation to IL-2. NK activity of the umbilical blood was significantly increased by melatonin and sodium selenite, but was not affected by NAC and quercetin. These data suggest that antioxidants are able to protect umbilical blood mononuclear cells against oxygen stress and affect oxygen stress mediated immune function inhibition of umbilical blood. These results will supply new experimental data for using umbilical blood to treat diseases.
我们之前已报道过健康足月儿的DNA氧化损伤和氧应激情况。本研究的目的是探讨褪黑素、亚硒酸钠、N-乙酰-L-半胱氨酸和槲皮素对脐血的抗氧化和免疫调节作用。采用单细胞凝胶电泳（彗星）试验检测DNA氧化损伤。通过³[H]-胸腺嘧啶核苷摄取法和MTT法检测脐血中淋巴细胞增殖和自然杀伤（NK）活性。彗星试验结果显示，褪黑素、N-乙酰-L-半胱氨酸（NAC）和槲皮素具有保护作用，而亚硒酸钠则具有保护和损伤作用。褪黑素、亚硒酸钠、NAC和槲皮素能显著促进淋巴细胞对白细胞介素-2的增殖。褪黑素和亚硒酸钠可显著提高脐血的NK活性，但NAC和槲皮素对其无影响。这些数据表明，抗氧化剂能够保护脐血单个核细胞免受氧应激影响，并影响氧应激介导的脐血免疫功能抑制。这些结果将为利用脐血治疗疾病提供新的实验数据。
