Ai Ping, Zheng Jian-Quan
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850.
Sheng Li Ke Xue Jin Zhan. 2005 Apr;36(2):125-9.
The patch-clamp technique, a dominant technique in cellular electrophysiology, is always being regarded as the gold standard for ion channel research. Application of the patch-clamp technique can demonstrate the existences of ion channels and provide valuable information for ion channels, including their electrophysiological properties, molecular structures and the mechanism of drug action. Genomics and proteomics research has showed that the development of drugs for ion channel target would be very promising in future. In recent years, numerous improvements have been achieved, which will facilitate the application of patch clamp technique in drug high-throughput screening. These techniques will break through the bottleneck in the development of drugs aiming at ion channels. New advancements in patch-clamp technique and application in drug high-throughput screening are reviewed in the paper.
膜片钳技术是细胞电生理学中的主导技术，一直被视为离子通道研究的金标准。膜片钳技术的应用能够证实离子通道的存在，并为离子通道提供有价值的信息，包括其电生理特性、分子结构以及药物作用机制。基因组学和蛋白质组学研究表明，针对离子通道靶点的药物开发在未来将非常有前景。近年来，已经取得了许多改进，这将促进膜片钳技术在药物高通量筛选中的应用。这些技术将突破针对离子通道药物开发的瓶颈。本文综述了膜片钳技术的新进展及其在药物高通量筛选中的应用。
