Khan Javaid Shahbaz, Imamoto Yasushi, Yamazaki Yoichi, Kataoka Mikio, Tokunaga Fumio, Terazima Masahide
Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
Anal Chem. 2005 Oct 15;77(20):6625-9. doi: 10.1021/ac050559n.
A new type of biosensor is presented for the first time. This method is based on the diffusion measurement in the time domain by the transient grating method. As the first demonstration of this new method, various intermolecular interactions with a reaction intermediate of photoactive yellow protein, such as the protein-protein interaction, protein-DNA interaction, and protein-small molecule binding are detected. The characteristic advantages and limitations are summarized.
首次提出了一种新型生物传感器。该方法基于瞬态光栅法在时域中的扩散测量。作为这种新方法的首次演示，检测了与光活性黄色蛋白反应中间体的各种分子间相互作用，如蛋白质-蛋白质相互作用、蛋白质-DNA相互作用和蛋白质-小分子结合。总结了其独特的优点和局限性。
