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[关于动物园反刍动物粪便样本中副结核分枝杆菌存在情况的细菌学研究]

[Bacterial studies on the occurrence of Mycobacterium paratuberculosis in fecal samples of zoo ruminants].

作者信息

Weber A, Gürke R, Bauer K, Schreyer K

机构信息

Landesuntesuchungsamt für das Gesundheitswesen Nordbayern, Nürnberg.

出版信息

Berl Munch Tierarztl Wochenschr. 1992 May 1;105(5):161-4.

PMID:1622380
Abstract

Mycobacterium (M.) paratuberculosis was isolated from fecal samples of 3 (21.4%) from 14 mouflons, of 10 (20.4%) from 49 dwarf goats, of 5 (14.3%) from 35 Cameroon sheep and of 1 (9.1%) from 11 alpine ibex. M. paratuberculosis could not found by cultural method in fecal samples of 22 Pinzgauer goats, of 15 bantengs, of 9 wild goats, of 9 skuddens, of 6 four-horned sheep, of 3 red-head sheep, and of 1 chamois. From all 19 animals with cultural positive fecal samples complement binding antibodies against M. paratuberculosis could not be found in the corresponding serum samples. The results confirm that M. paratuberculosis is more frequently in small zoo ruminants than up to now was suspected. The cultural examination of fecal samples has been proved to be a better method for detecting animal excretors than serological investigations by means of the complement fixation test.

摘要

从14只摩弗伦羊的粪便样本中分离出3株(21.4%)副结核分枝杆菌,从49只侏儒山羊的粪便样本中分离出10株(20.4%),从35只喀麦隆绵羊的粪便样本中分离出5株(14.3%),从11只阿尔卑斯野山羊的粪便样本中分离出1株(9.1%)。通过培养方法,在22只平茨高尔山羊、15只爪哇野牛、9只野山羊、9只斯库登羊、6只四角羊、3只红头羊和1只羚羊的粪便样本中未发现副结核分枝杆菌。在所有粪便样本培养呈阳性的19只动物的相应血清样本中,均未发现抗副结核分枝杆菌的补体结合抗体。结果证实,副结核分枝杆菌在小型动物园反刍动物中的感染比目前怀疑的更为频繁。粪便样本的培养检查已被证明是一种比通过补体结合试验进行血清学调查更好的检测动物排泄物的方法。

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Characterization of genetic differences between Mycobacterium avium subsp. paratuberculosis type I and type II isolates.鸟分枝杆菌副结核亚种I型和II型分离株之间遗传差异的特征分析
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Molecular characterization of Mycobacterium paratuberculosis isolates from sheep, goats, and cattle by hybridization with a DNA probe to insertion element IS900.通过与插入元件IS900的DNA探针杂交对来自绵羊、山羊和牛的副结核分枝杆菌分离株进行分子特征分析。
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