Xi Zhiyong, Khoo Cynthia C H, Dobson Stephen L
Department of Entomology, University of Kentucky, Lexington, KY 40546, USA.
Science. 2005 Oct 14;310(5746):326-8. doi: 10.1126/science.1117607.
A proposed strategy to aid in controlling the growing burden of vector-borne disease is population replacement, in which a natural vector population is replaced by a population with a reduced capacity for disease transmission. An important component of such a strategy is the drive system, which serves to spread a desired genotype into the targeted field population. Endosymbiotic Wolbachia bacteria are potential transgene drivers, but infections do not naturally occur in some important mosquito vectors, notably Aedes aegypti. In this work, stable infections of wAlbB Wolbachia were established in A. aegypti and caused high rates of cytoplasmic incompatibility (that is, elimination of egg hatch). Laboratory cage tests demonstrated the ability of wAlbB to spread into an A. aegypti population after seeding of an uninfected population with infected females, reaching infection fixation within seven generations.
一种有助于控制虫媒疾病日益增长负担的策略是种群替换，即自然病媒种群被疾病传播能力降低的种群所取代。这种策略的一个重要组成部分是驱动系统，其作用是将所需的基因型传播到目标野外种群中。内共生沃尔巴克氏体细菌是潜在的转基因驱动因子，但在一些重要的蚊子病媒中，尤其是埃及伊蚊，自然情况下不会发生感染。在这项研究中，在埃及伊蚊中建立了稳定的wAlbB沃尔巴克氏体感染，并导致了高比率的细胞质不亲和性（即卵孵化失败）。实验室笼子试验证明，在用感染的雌性蚊子接种未感染种群后，wAlbB能够传播到埃及伊蚊种群中，并在七代内达到感染固定状态。
