Tessitore L, Baccino F M
Boll Soc Ital Biol Sper. 1979 Jul 15;55(12):1136-40.
Recently investigators reported ultrastructural modifications of rat liver lysosomes which probably correlate with an increased level of blood plasma glucagon in streptozotocin-diabetes. We are investigating whether biochemical changes occur in this condition. Lysosome fragility is increased in the hepatocytes of streptozotocin-diabetic rats. Moreover the plasma activity of two glycosidases, B-N-acetylglucosaminidase and B-galactosidase, is markedly increased in streptozotocin-treated rats. Both these changes are largely prevented by insulin treatment. These findings support the idea that the morphologic and biochemical modifications of the hepatocytes, which are observed in experimental diabetes, involve the lysosomes showing an increased autophagic activity which is probably connected with enhanced liver protein catabolism.
最近，研究人员报告了大鼠肝脏溶酶体的超微结构改变，这可能与链脲佐菌素诱导的糖尿病中血浆胰高血糖素水平升高有关。我们正在研究在这种情况下是否会发生生化变化。链脲佐菌素诱导的糖尿病大鼠肝细胞中溶酶体脆性增加。此外，在链脲佐菌素处理的大鼠中，两种糖苷酶，β-N-乙酰氨基葡萄糖苷酶和β-半乳糖苷酶的血浆活性显著增加。胰岛素治疗在很大程度上预防了这两种变化。这些发现支持了这样一种观点，即在实验性糖尿病中观察到的肝细胞形态和生化改变涉及溶酶体，溶酶体自噬活性增加，这可能与肝脏蛋白质分解代谢增强有关。
