Lazár D, Tomek P, Ilík P, Naus J
Faculty of Science, Department of Experimental Physics, Palacký University, tr. Svobody 26, 771 46, Olomouc, Czech Republic,
Photosynth Res. 2001;68(3):247-57. doi: 10.1023/A:1012973402023.
Several methods for determination of the antenna heterogeneity of Photosystem II from fluorescence rise curves measured with DCMU have been developed so far. Using these methods, two, three or four types of Photosystem II with respect to the antenna heterogeneity were determined. However, the accuracy of some of these methods is under debate. Here, we present a new method for the determination of the antenna heterogeneity of Photosystem II. The method is based on direct simultaneous fitting of several fluorescence rise curves measured with DCMU at different intensities of light excitation. As several curves measured under different light conditions are fitted simultaneously by the same model, reliability and accuracy in determination of model parameters increase. Our method was applied to two plant materials with different structure of the thylakoid membrane: wheat leaves and cells of green alga Chlamydomonas reinhardtii.
迄今为止，已经开发了几种通过用敌草隆（DCMU）测量的荧光上升曲线来测定光系统II天线异质性的方法。使用这些方法，确定了两种、三种或四种关于天线异质性的光系统II类型。然而，其中一些方法的准确性仍存在争议。在此，我们提出一种测定光系统II天线异质性的新方法。该方法基于对在不同光激发强度下用敌草隆测量的几条荧光上升曲线进行直接同时拟合。由于在不同光照条件下测量的几条曲线由同一模型同时拟合，模型参数测定的可靠性和准确性提高。我们的方法应用于两种具有不同类囊体膜结构的植物材料：小麦叶片和绿藻莱茵衣藻的细胞。
