Gregoraszczuk Ewa Lucia, Zemla Malogorzata, Ptak Anna, Grabic Roman
Laboratory of Physiology and Toxicology of Reproduction, Department of Animal Physiology, Institute of Zoology, Jagiellonian University, Kraków, Poland.
Endocr Regul. 2005 Jun;39(2):33-41.
To compare the effects on ovarian function of low chlorinated polychlorinated biphenyls (PCBs) mixture generally distributed at a faster rate and metabolised to a greater extent (Delor 103) with high chlorinated PCBs mixture which are accumulated in large amounts in biological samples (Delor 106).
Theca interna and granulosa cells were isolated from prepubertal porcine follicles and initially cultured in M199/CS without test compounds for 24 hrs to allow for cell attachment to the wells. After 24 h, the medium was discarded and 0.3 ml of fresh M199 alone was added to the control culture, while Delor 103 or Delor 106 at concentrations of 0.02, 0.2 or 2 ng/ml medium were added to the cultures. For analysis of steroid levels, the media were collected after 24 hrs (single exposure) or 72 hrs (repeated exposure) and frozen at--20 degrees C. Additionally, after 72 hrs, LDL test was used to show the action of both mixtures on the cell viability and cells were used for the measurement of caspase-3 activity.
An increase in testosterone secretion after the single exposure and strong stimulatory action on estradiol secretion was noted after permanent treatment with Delor 103. In Delor 106 treated cultures, antiestrogenic action after the single exposure and strong estrogenic action in permanently treated cells was observed. Both mixtures had no effect on cell viability and apoptosis.
By stimulation of estradiol secretion from ovarian follicles in prepubertal animals, PCB mixture could be responsible for premature development of follicles and in consequence, premature sexual maturation.
比较低氯多氯联苯（PCBs）混合物（Delor 103，通常以更快的速度分布且代谢程度更高）与高氯PCBs混合物（Delor 106，在生物样品中大量蓄积）对卵巢功能的影响。
从青春期前猪卵泡中分离出卵泡内膜细胞和颗粒细胞，最初在不含测试化合物的M199/CS中培养24小时，以使细胞附着于孔中。24小时后，弃去培养基，向对照培养物中仅添加0.3 ml新鲜M199，而向培养物中添加浓度为0.02、0.2或2 ng/ml培养基的Delor 103或Delor 106。为了分析类固醇水平，在24小时（单次暴露）或72小时（重复暴露）后收集培养基并在-20℃下冷冻。此外，72小时后，使用低密度脂蛋白（LDL）测试来显示两种混合物对细胞活力的作用，并将细胞用于测量半胱天冬酶-3活性。
单次暴露后睾酮分泌增加，用Delor 103长期处理后对雌二醇分泌有强烈的刺激作用。在Delor 106处理的培养物中，观察到单次暴露后的抗雌激素作用和长期处理细胞后的强烈雌激素作用。两种混合物对细胞活力和细胞凋亡均无影响。
通过刺激青春期前动物卵巢卵泡分泌雌二醇，PCBs混合物可能导致卵泡过早发育，进而导致性早熟。
