Karagenc Tulin Ilhan, Pasa Serdar, Kirli Gulcan, Hosgor Murat, Bilgic Huseyin Bilgin, Ozon Yavuz Hakan, Atasoy Abidin, Eren Hasan
Department of Parasitology, Faculty of Veterinary Medicine, University of Adnan Menderes, Aydin, Turkey.
Vet Parasitol. 2006 Jan 30;135(2):113-9. doi: 10.1016/j.vetpar.2005.08.007. Epub 2005 Oct 17.
Canine hepatozoonosis is caused by the tick-borne protozoon Hepatozoon spp. The prevalence of the infection in the Aegean coast of Turkey was investigated by examination of blood smear parasitology and polymerase chain reaction (PCR) using blood samples from 349 dogs collected from Central Aydin, Kusadasi, Selcuk, Central Manisa, Bodrum and Marmaris within the Aegean coast of Turkey. The indirect fluorescent antibody test (IFAT) for the detection of Hepatozoon canis antibodies was also used to detect the exposure rate to H. canis. PCR amplifying a 666bp fragment of 18S rRNA gene of Hepatozoon spp. was used in the epidemiological survey. The prevalence of Hepatozoon spp. infection was 10.6% by blood smear parasitology and 25.8% by PCR. IFAT revealed that 36.8% of serum samples were positive for antibodies reactive with Hepatozoon spp. The PCR products of 18S rRNA gene of Hepatozoon spp. isolated from six infected dogs, one isolate originating from each of the six different locations, were sequenced. The results of sequence analysis indicate that they are closely related to Indian and Japanese isolates of H. canis. This is the first epidemiological study on the prevalence of H. canis infection in the dog, in Turkey.
犬肝簇虫病由蜱传播的原虫肝簇虫属（Hepatozoon spp.）引起。通过对从土耳其爱琴海岸的艾登市中心、库萨达斯、塞尔丘克、马尼萨市中心、博德鲁姆和马尔马里斯采集的349份犬血样进行血涂片寄生虫学检查和聚合酶链反应（PCR），调查了土耳其爱琴海岸该感染的患病率。还使用检测犬肝簇虫抗体的间接荧光抗体试验（IFAT）来检测犬对犬肝簇虫的暴露率。在流行病学调查中使用PCR扩增肝簇虫属18S rRNA基因的666bp片段。通过血涂片寄生虫学检查，肝簇虫属感染的患病率为10.6%，通过PCR为25.8%。IFAT显示36.8%的血清样本对与肝簇虫属反应的抗体呈阳性。对从六只感染犬分离出的肝簇虫属18S rRNA基因的PCR产物进行测序，这六个分离株分别来自六个不同地点。序列分析结果表明，它们与印度和日本的犬肝簇虫分离株密切相关。这是土耳其首次关于犬感染犬肝簇虫患病率的流行病学研究。
