[鼻咽上皮细胞中的调节性容积减小及其机制]

[Regulatory volume decrease and its mechanism in nasopharyngeal epithelial cells].

作者信息

Sun Xue Rong, Chen Li Xin, Mao Jian Wen, Zhu Lin Yan, Nie Si Huai, Zhong Ping, Li Pan, Wang Li Wei

机构信息

Laboratory of Physiology, Guangdong Medical College, Zhanjiang.

出版信息

Shi Yan Sheng Wu Xue Bao. 2005 Aug;38(4):353-8.

PMID:16231703

Abstract

To investigate regulatory volume decrease (RVD) and its mechanism in primary-culturing fetal human nasopharyngeal epithelial cells, living cell imaging technique was employed to detect the volume changes following exposure to hypotonic solution, and blockage of Cl- channels was used to clarify the role of Cl- channels in RVD. The results showed that extracellular hypotonic treatment swelled the cells and induced RVD. 47% hypotonic solution (160 mOsmol/L) swelled the cell by 144.7% and induced 38.7% recovery of cell volume within 20 min. RVD was correlated negatively to the extracellular osmolarity (r=-0.99, P<0.05) and positively to the swelling volume(r=0.99, P<0.05) in "S" shape, respectively. Chloride channel blockers, tamoxfen (20 micromol/ L), ATP (10 mmol/L) and NPPB (100 micromol/L), inhibited RVD by 100%, 76.3% and 62.7% (P< 0.01), respectively. The results indicated that primary-culturing fetal human nasopharyngeal epithelial cells are capable of RVD. Cl- efflux through Cl- channels is the key mechanism of RVD.

摘要

为研究原代培养的人胎儿鼻咽上皮细胞的调节性容积减小（RVD）及其机制，采用活细胞成像技术检测细胞暴露于低渗溶液后的容积变化，并通过阻断氯离子通道来阐明氯离子通道在RVD中的作用。结果显示，细胞外低渗处理使细胞肿胀并诱导了RVD。47%的低渗溶液（160 mOsmol/L）使细胞肿胀了144.7%，并在20分钟内诱导细胞容积恢复了38.7%。RVD分别与细胞外渗透压呈负相关（r = -0.99，P < 0.05），与肿胀容积呈正相关（r = 0.99，P < 0.05），呈“S”形关系。氯离子通道阻滞剂他莫昔芬（20 μmol/L）、ATP（10 mmol/L）和NPPB（100 μmol/L）分别使RVD抑制了100%、76.3%和62.7%（P < 0.01）。结果表明，原代培养的人胎儿鼻咽上皮细胞具有RVD能力。氯离子通过氯离子通道外流是RVD的关键机制。