Shi Lu Ping, Zang Yi Min, Hou Xiao Li, Wang Jun
Department of Physiology, Capital Medical University, Beijing 100069, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2008 Aug;24(3):356-60.
To observe the regulatory volume decrease (RVD) process of human intestine cells and investigate its ion channel mechanism.
Cultured human intestine cells were exposed to hypotonic solution and the cell volume was measured using Coulter Counter System. RT-PCR was explored to detect the mRNA expression of Ca(2+) -activated K+ channel.
Human intestine cells showed a RVD process and this process could be blocked by Cl- channel blocker NPPB and K+ channel blocker TEA. Further results demonstrated the subtype of K+ channel involved in RVD was an intermediate-conductance, Ca(2+) -activated K+ channel (IK) because of its high sensitivity to clotrimazole. RT-PCR results also showed the expression of IK in this cell line.
The RVD process of intestine cell was dependent on the parallel activation of Cl- channel and K+ channel. The subtype of K+ channel in volved in the RVD process was IK channel.
观察人肠细胞的调节性容积减小（RVD）过程，并探讨其离子通道机制。
将培养的人肠细胞置于低渗溶液中，使用库尔特计数器系统测量细胞体积。采用逆转录聚合酶链反应（RT-PCR）检测钙激活钾通道的mRNA表达。
人肠细胞呈现RVD过程，该过程可被氯离子通道阻滞剂NPPB和钾通道阻滞剂TEA阻断。进一步结果表明，参与RVD的钾通道亚型为中电导钙激活钾通道（IK），因为其对克霉唑高度敏感。RT-PCR结果也显示该细胞系中有IK的表达。
肠细胞的RVD过程依赖于氯离子通道和钾通道的并行激活。参与RVD过程的钾通道亚型为IK通道。
