Herschkovitz Yoav, Lerner Anat, Davidov Yaacov, Okon Yaacov, Jurkevitch Edouard
Department of Plant Pathology and Microbiology, and The Otto Warburg Minerva Center for Agricultural Biotechnology, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
Environ Microbiol. 2005 Nov;7(11):1847-52. doi: 10.1111/j.1462-2920.2005.00926.x.
Positive response of plant species to plant growth-promoting rhizobacteria have led to an increased interest in their use as bacterial inoculants. However, the introduction of exogenous bacteria into natural ecosystems may perturb bacterial populations within the microbial community and lead to the disruption of indigenous populations performing key functional roles. In this study the effect of Azospirillum brasilense inoculation on maize (Zea mays) rhizosphere Actinobacteria, Bacteroidetes, alpha-Proteobacteria, Pseudomonas and Bdellovibrio spp. was assessed using a polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) approach in conjunction with group-specific primers. The DGGE fingerprints analysis revealed that the introduction of A. brasilense did not alter or disrupt the microbial system at the group-specific level. However, some communities such as the alpha-Proteobacteria and Bdellovibrio were influenced by plant age while the other bacterial groups remained unaffected. Based on these as well as previous data, it can be inferred that inoculation with A. brasilense does not perturb the natural bacterial populations investigated.
植物物种对促进植物生长的根际细菌的积极反应，引发了人们对将其用作细菌接种剂的兴趣增加。然而，将外源细菌引入自然生态系统可能会扰乱微生物群落中的细菌种群，并导致执行关键功能的本土种群受到破坏。在本研究中，使用聚合酶链反应（PCR）-变性梯度凝胶电泳（DGGE）方法结合组特异性引物，评估了巴西固氮螺菌接种对玉米（Zea mays）根际放线菌、拟杆菌、α-变形菌、假单胞菌和蛭弧菌属的影响。DGGE指纹分析表明，巴西固氮螺菌的引入在组特异性水平上并未改变或破坏微生物系统。然而，一些群落，如α-变形菌和蛭弧菌，受植物年龄的影响，而其他细菌组则不受影响。基于这些以及先前的数据，可以推断，接种巴西固氮螺菌不会扰乱所研究的自然细菌种群。
