Kobayashi Gota, Fujii Kaori, Serizawa Masakuni, Yamamoto Hiroki, Sekiguchi Junichi
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda, Nagano 386-8567, Japan.
J Biosci Bioeng. 2002;93(1):15-9. doi: 10.1016/s1389-1723(02)80047-0.
A small cell wall-binding domain (CWB(c)) of the Bacillus subtilis peptidoglycan hydrolase CwlC fused to B. subtilis lipase B was able to be localized on the cell wall of B. subtilis. With the aim of developing an efficient lipid-hydrolyzing bacterium with two different lipases on the cell surface, plasmids possessing genes for LipB-CWB(c) and CWB(b)-CutL (the cell wall-binding domain of CwlB fused to the Aspergillus oryzae lipolytic enzyme CutL) were constructed. B. subtilis harboring these plasmids accumulated considerable amounts of both lipases on the cell surface.
将枯草芽孢杆菌肽聚糖水解酶CwlC的一个小细胞壁结合结构域（CWB(c)）与枯草芽孢杆菌脂肪酶B融合后，能够定位于枯草芽孢杆菌的细胞壁上。为了开发一种在细胞表面具有两种不同脂肪酶的高效脂质水解细菌，构建了携带LipB-CWB(c)和CWB(b)-CutL（CwlB的细胞壁结合结构域与米曲霉脂肪分解酶CutL融合）基因的质粒。携带这些质粒的枯草芽孢杆菌在细胞表面积累了大量的两种脂肪酶。
