由糖醛酸产生的热降解产物促进长春花细胞产生5'-磷酸二酯酶。
Production of 5'-phosphodiesterase by Catharanthus roseus cells promoted by heat-degraded products generated from uronic acid.
作者信息
Akimoto-Tomiyama Chiharu, Aoyagi Hideki, Ozawa Tetsuo, Tanaka Hideo
机构信息
Institute of Applied Biochemistry, University of Tsukuba, Tsuhtba, Ibaraki 305-8572, Japan.
出版信息
J Biosci Bioeng. 2002;94(2):154-9. doi: 10.1263/jbb.94.154.
Polyalginate was autoclaved at 121 degrees C for 20 min and its molecular weight distribution was analyzed. The autoclaved alginate yielded alginate polymer, oligomer and heat degraded products (HDPs). Each of the separated substances promoted 5'-phosphodiesterase (5'-PDase) production in suspension culture of Catharanthus roseus cells. HDPs could also be generated from other uronic acids (galacturonic acid and glucuronic acid) by autoclave treatment. The most effective substance in the HDPs was isolated and characterized as trans-4,5-dihydroxy-2-cyclopenten-1-one (DHCP). The optimal conditions for DHCP production were also established (autoclaving 1 mg/ml monogalacturonic acid [pH 2] at 121 degrees C for 2 h). A combination of oligo-alginate (below 4 kDa) and HDPs significantly promoted the production of 5'-PDase in C. roseus. Based on the above results, a novel alginate complex that gave a 44-fold increase in 5'-PDase production by C. roseus was developed.
将海藻酸多聚体在121℃下高压灭菌20分钟，并分析其分子量分布。经高压灭菌的海藻酸盐产生了海藻酸盐聚合物、低聚物和热降解产物（HDPs）。每种分离出的物质都能促进长春花细胞悬浮培养中5'-磷酸二酯酶（5'-PDase）的产生。通过高压灭菌处理，其他糖醛酸（半乳糖醛酸和葡萄糖醛酸）也能产生HDPs。从HDPs中分离出最有效的物质，并将其鉴定为反式-4,5-二羟基-2-环戊烯-1-酮（DHCP）。还确定了DHCP产生的最佳条件（在121℃下将1mg/ml的单半乳糖醛酸[pH 2]高压灭菌2小时）。低聚海藻酸盐（低于4kDa）和HDPs的组合显著促进了长春花中5'-PDase的产生。基于上述结果，开发了一种新型海藻酸盐复合物，它能使长春花中5'-PDase的产量提高44倍。
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